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| MBTL5043 | Essential Technicsin Molecular Biology | 3+0+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of BIOTECHNOLOGY (INTERDISCIPLINARY) | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Prof. Dr. Remziye NALÇACIOĞLU | | Co-Lecturer | Prof. Dr. Zihni Demirbağ | | Language of instruction | | | Professional practise ( internship ) | None | | | | The aim of the course: | | Learning essential methods in molecular biology |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Learn the basic techniques in molecular biology | 1,2 | 1,5, | | PO - 2 : | Able to modify molecular techniques for own work | 1,2 | 1,5, | | PO - 3 : | Able to follow current techniques in the literature | 1,2 | 1,5, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Basic principals in molecular biology; Cell culture; Centrifugation; Microscopy; Molecular biology and bioinformatics; recombinant DNA and genetic analysis; Immunochemical techniques; Protein structure, purification, charecterisation and analysis; Mass spectroscopy; Electrophoresis; Chromatography; Spectroscopy; Radioisotope techniques; Enzymes; Principals of clinical biochemistry; Cell membrane reseptors and cell signaling; Drug discovery and development |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Basic principals in molecular biology; | | | Week 2 | Cell culture; | | | Week 3 | Centrifugation; | | | Week 4 | Microscopy; | | | Week 5 | Molecular biology and bioinformatics; | | | Week 6 | Recombinant DNA and genetic analysis; | | | Week 7 | Immunochemical techniques; | | | Week 8 | Protein structure, purification, charecterisation and analysis; Mass spectroscopy; | | | Week 9 | First Exam | | | Week 10 | Electrophoresis; Chromatography; | | | Week 11 | Spectroscopy; Radioisotope techniques; | | | Week 12 | Seminer Presentation | | | Week 13 | Enzymes; Principals of clinical biochemistry; | | | Week 14 | Cell membrane receptors and cell signaling; | | | Week 15 | Drug discovery and development | | | Week 16 | Final exam | | | |
| 1 | Surzycki, S. 2000. Basic Techniques in Molecular Biology. Springer Lab Manual. | | | 2 | Temizkan, G ve Arda, N. 2021. Temel ve İleri Moleküler Biyoloji Yöntemleri Genomik ve Proteomik Analizler 2. Baskı. Nobel Tıp Kitapevleri | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 22/11/2021 | 1 | 20 | | Presentation | 12 | 13/12/2021 | 1 | 30 | | End-of-term exam | 16 | 15/01/2022 | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 13 | 39 | | Arasınav için hazırlık | 1 | 13 | 13 | | Arasınav | 1 | 1 | 1 | | Ödev | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 1 | 13 | 13 | | Dönem sonu sınavı | 1 | 1 | 1 | | Total work load | | | 68 |
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