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| MBGL5037 | Molecular Biology of The Gene | 3+0+0 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Dr. Öğr. Üyesi Uğur UZUNER | | Co-Lecturer | | | Language of instruction | | | Professional practise ( internship ) | None | | | | The aim of the course: | | This course is to give an in-depth knowledge of the fundamentals of molecular biology and key molecules; From DNA to protein production, to provide a comprehensive description of the Central Dogma process and related key molecules. Make an update on the latest developments in current molecular biology methods; Provide comprehensive information on DNA replication, transcription, regulatory molecules and mechanisms during translation; The course aims to explain the maintenance and repair mechanisms of the genome and provide explanatory information about the newly developed genome engineering strategies. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Understands the functioning of genes, distinguish between prokaryotic and eukaryotic gene structures | 1,2,3,4,5,6,7 | 1,5,6 | | PO - 2 : | Comprehend the components and specific roles of Central Dogma | 1,2,3,4,5,6,7 | 1,5,6 | | PO - 3 : | Gain theoretical competence in DNA regulation and genetic engineering | 1,2,3,4,5,6,7 | 1,5,6 | | PO - 4 : | Gain comprehensive knowledge in the field of genetic engineering | 1,2,3,4,5,6,7 | 1,5,6 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Fundamentals and molecular biology of gene structures will be discussed. Students will gain in-depth knowledge of nucleic acid structure, molecular genetics and biochemistry of transcription and protein synthesis. Based on this fiction, students will explore gene regulation mechanisms in prokaryotes and eukaryotes. Topics include: Introduction to Gene Molecular Biology, Structure and Study of Macromolecules: DNA; Structure and Study of Macromolecules: RNA; Structure and Study of Macromolecules: Proteins; Molecular Biology Techniques; Maintenance and maintenance of genome structure; Replication of DNA; DNA Repair and Homologous Recombination; Transcription Mechanisms, RNA Insertion and Translation; Transcriptional Regulation in Prokaryotes and Eukaryotes; Regulatory RNAs; Gene regulation, Development and Evolution. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Macromolecules | | | Week 2 | The Importance of Weak and Strong Chemical Bonds | | | Week 3 | Structure of DNA | | | Week 4 | The Structure and Versatility of RNA | | | Week 5 | The Structure of Proteins | | | Week 6 | Techniques of Molecular Biology | | | Week 7 | Midterm Exam | | | Week 8 | Genome Structure, Chromatin, and the Nucleosome | | | Week 9 | The Replication of DNA | | | Week 10 | The Mutability and Repair of DNA | | | Week 11 | Homologous Recombination at the Molecular Level | | | Week 12 | EXPRESSION OF THE GENOME | | | Week 13 | RNA Splicing and Translation | | | Week 14 | Seminar / Transcriptional Regulation in Prokaryotes and Eukaryotes | | | Week 15 | Seminar / Regulatory RNAs | | | Week 16 | Final Exam | | | |
| 1 | James D. Watson. Alexander Gann. Stephen Harrison. (2013). Molecular Biology of the Gene. COLD SPRING HARBOR LABORATORY PRESS | | | |
| 1 | Glaucia C. Pereira (2022). Gene, Drug, and Tissue Engineering. Methods in Molecular Biology, 257. Humana Press City: New York | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 7 | 14/11/2019 | 3 | 30 | | Presentation | 14
15 | 19/12/2019 | 6 | 10 | | Homework/Assignment/Term-paper | 14 | 19/12/2019 | 3 | 10 | | End-of-term exam | 17 | 03/01/2020 | 3 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 3 | 16 | 48 | | Arasınav için hazırlık | 3 | 6 | 18 | | Arasınav | 3 | 1 | 3 | | Uygulama | 3 | 4 | 12 | | Ödev | 3 | 6 | 18 | | Proje | 3 | 5 | 15 | | Kısa sınav | 3 | 6 | 18 | | Dönem sonu sınavı için hazırlık | 3 | 6 | 18 | | Dönem sonu sınavı | 3 | 1 | 3 | | Total work load | | | 195 |
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