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| MBGL5039 | The Methods for Epigenetics | 3+0+0 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Doç. Dr. Selcen ÇELİK UZUNER | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | This course aims to understand which methods are suitable for the detection of epigenetic modifications in DNA and histones, to realize the advantages and disadvantages of each method, and to enable students to reach a level of knowledge that can determine the methods they will choose in practice. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Defines, classifies and understands the biological significance of epigenetic modifications. | 3,5,8 | 1,3, | | PO - 2 : | Distinguish the techniques based on spectrometric, antibody-based and sequencing mapping of epigenetic modifications in DNA according to their purposes. | 3,5,8 | 1,3, | | PO - 3 : | Classify the techniques used to detect epigenetic modifications in histones. | 3,5,8 | 1,3, | | PO - 4 : | Learns the methods that can detect epigenetic modifications in RNA. | 3,5,8 | 1,3, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| This course includes learning the classification, scope and usage areas of current and improved techniques used in detecting epigenetic modifications in DNA and histone proteins at the gene and genome level. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Introduction to Epigenetics - 1 | | | Week 2 | Introduction to Epigenetics - 2 | | | Week 3 | Detection and Quantification of DNA Modifications Using Mass Spectrometry and Capillary Gel Electrophoresis | | | Week 4 | Assessing the Levels and Spatial Distribution of DNA Modifications Using Antibody-Based Approaches | | | Week 5 | Mapping DNA Modifications | | | Week 6 | Applications of Genome Targeting Methods in Epigenetics | | | Week 7 | Analysis of DNA Modifications Sequencing Datasets | | | Week 8 | Detection of histone modifications - 1 | | | Week 9 | Mid term exam | | | Week 10 | Detection of histone modifications - 2 | | | Week 11 | Detection of histone modifications - 3 | | | Week 12 | Student presentations -1 | | | Week 13 | Student presentations -2 | | | Week 14 | Detection of RNA modifications -1 | | | Week 15 | Detection of RNA modifications -2 | | | Week 16 | Conclusion - Detection of epigenetic modifications at genome and gene level | | | |
| 1 | DNA Modifications in Methods and Protocols, Part of the Methods in Molecular Biology (MIMB) book series, 2198, pp 217-226, Springer, 2021 | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | | 2 | 30 | | In-term studies (second mid-term exam) | 9 | | 2 | 20 | | End-of-term exam | 16 | | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 2 | 3 | 6 | | Arasınav için hazırlık | 5 | 6 | 30 | | Arasınav | 2 | 1 | 2 | | Ödev | 2 | 1 | 2 | | Dönem sonu sınavı için hazırlık | 8 | 13 | 104 | | Dönem sonu sınavı | 3 | 1 | 3 | | Total work load | | | 189 |
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