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| MBGL7070 | Principles of Gene Manipulation | 3+0+0 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Doç. Dr. Halil İbrahim GÜLER | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | The aim of this course is to educate biology and molecular biology students, as well as other life sciences and bioengineering students, who are not biologically rooted, to have knowledge of gene technology and recombinant DNA technology. The basic principles of gene manipulation, cloning strategies, gene transfer studies, basic biology of plasmid and phage vectors, and knowledge of students about sequencing and mutagenesis studies are basic principles. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Basic principles of gene manipulation | 2,3,5 | 1 | | PO - 2 : | Basic techniques in manipulation | 2,3,5 | 1 | | PO - 3 : | Cloning strategies | 2,3,5 | 1 | | PO - 4 : | Gene transfer to various cells | 2,3,5 | 1 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Content of the course; to learn the basic principles of gene manipulation, to have knowledge about basic techniques used in molecular biology laboratory, to examine the structure of vectors and phagemids used in recombinant DNA studies, to have information about the analysis of DNA sequences, tp learn various cloning strategies |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Basic principles in gene manipulation | | | Week 2 | The role of E.coli | | | Week 3 | Agarose gel electrophoresis | | | Week 4 | Transformation | | | Week 5 | PCR | | | Week 6 | Digesting of DNA molecules | | | Week 7 | Ligation of of DNA molecules | | | Week 8 | Mid-term Exam | | | Week 9 | Biology of vector and plasmid | | | Week 10 | Cosmids, phages, other vectors | | | Week 11 | Cloning of large DNA fragments | | | Week 12 | Vectors used for special purposes | | | Week 13 | Screening strategies | | | Week 14 | Genome sequencing | | | Week 15 | Analysis of sequence data | | | Week 16 | Final Exam | | | |
| 1 | Principles of gene manipulation | | | |
| 1 | Öğretim ElemanInIn Ders notlarI | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | | 2 | 50 | | End-of-term exam | 16 | | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 2 | 14 | 28 | | Arasınav için hazırlık | 3 | 8 | 24 | | Arasınav | 2 | 1 | 2 | | Dönem sonu sınavı için hazırlık | 3 | 14 | 42 | | Dönem sonu sınavı | 2 | 1 | 2 | | Diğer 1 | 3 | 14 | 42 | | Diğer 2 | 3 | 14 | 42 | | Total work load | | | 224 |
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