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| MBGL5034 | Genetic and protein engineering | 3+0+0 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Dr. Öğr. Üyesi Uğur UZUNER | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | The aim of this course is to raise awareness of students about the latest developments in gene and protein engineering and current techniques. This approach also targets to contribute to the knowledge and development of students on agriculture, biotechnology, food&feed, health and medicine by unraveling the current practices and potentials. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Be aware of current applications in gene and protein engineering | 3,4,5,6 | 1,5 | | PO - 2 : | Recognize the functional structures of the genes and acquire critical elements | 3,4,5,6 | 1,5 | | PO - 3 : | Has knowledge about protein folding, functionality and engineering | 3,4,5,6 | 1,5 | | PO - 4 : | Design complex expression cassettes adaptable to biotechnology | 3,4,5,6 | 1,5 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| This course will cover topics such as introduction to recombinant DNA technologies, prokaryotic and eukaryotic gene structures, isolation and electrophoresis of nucleic acids and proteins, Modern PCR applications, Design of recombinant gene expression cassettes (vectors and hosts), Different transformation techniques in homologus and heterologous gene expression, Protein folding and 3 dimensional structure modeling , Bioinformatics and modern approaches in protein engineering, techniques for determining protein-protein and protein-DNA interactions, CRISPR in gene engineering, VIGS and RNAi mechanisms and gene therapy applications. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Introduction to recombinant DNA technologies | | | Week 2 | Prokaryotic and eukaryotic gene structures | | | Week 3 | Isolation and electrophoresis of nucleic acids and protein | | | Week 4 | Modern PCR applications (qPCR, RTPCR, overlapping PCR, Gibson assembly) | | | Week 5 | The design of recombinant gene expression cassettes (vectors and hosts) | | | Week 6 | Different transformation techniques in homologus and heterologous gene expression | | | Week 7 | Different approaches in gene and protein engineering | | | Week 8 | Mid Term | | | Week 9 | Protein folding and 3D structure modeling | | | Week 10 | Bioinformatics and modern approaches in protein engineering | | | Week 11 | Techniques for determining protein-protein and protein-DNA interactions | | | Week 12 | Gene engineering of genomes by using CRISPR-cas9 system | | | Week 13 | VIGS and RNAi mechanisms in gene engineering. | | | Week 14 | Student Seminars | | | Week 15 | Gene therapy applications | | | Week 16 | Final Exam | | | |
| 1 | Molecular Biology of the Gene. 7th Edition., JAMES D. WATSON et al., 2014/ CSH Press/ISBN-13: 978-0-321-76243-6 | | | |
| 1 | Protein Bioinformatics: From Sequence to Function. M. Michael Gromiha et al. 2010 / Elsevier, ISBN-13: 978-81-312-2297-3 | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | 18.11.2021 | 3 | 30 | | Presentation | 14 | 18.01.2022 | 3 | 20 | | End-of-term exam | 16 | 25.01.2022 | 3 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 3 | 14 | 42 | | Arasınav için hazırlık | 3 | 6 | 18 | | Arasınav | 3 | 1 | 3 | | Uygulama | 3 | 6 | 18 | | Ödev | 3 | 6 | 18 | | Proje | 3 | 6 | 18 | | Kısa sınav | 3 | 6 | 18 | | Dönem sonu sınavı için hazırlık | 3 | 6 | 18 | | Dönem sonu sınavı | 3 | 1 | 3 | | Total work load | | | 198 |
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