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| MBGL7071 | Real Time PCR Methods and Applications | 3+0+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Doç. Dr. Halil İbrahim GÜLER | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | The aim of this course is to educate biology and molecular biology students, as well as other life sciences and bioengineering students, who are not biologically rooted, to have knowledge of basic topics about Real-Time applications and to have a general understanding of Real-Time methods. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Basic principles of Real-Time PCR | 2,5 | 1, | | PO - 2 : | Basic techniques in relative quantification | 2,5 | 1, | | PO - 3 : | Performing a RT-PCR experiment | 2,5 | 1, | | PO - 4 : | Designing primers and probes for qPCR | 2,5 | 1, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Content of the course; to learn the basic principles of Real Time PCR, to have knowledge about basic protocols used in RT-PCR experiments, to have information about Standart curves, data reporting, methods of normalization and syber green technology, to have knowledge about probe and primer design for qPCR. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | An introduction to real-time PCR | | | Week 2 | Detection chemistries used in real-time PCR | | | Week 3 | Performing a real-time RT-PCR experiment | | | Week 4 | Standard curves | | | Week 5 | Preliminary analysis | | | Week 6 | Data reporting and statistics | | | Week 7 | Relative quantification | | | Week 8 | Mid-term exam | | | Week 9 | Relative quantification data analysis and software applications | | | Week 10 | Normalization | | | Week 11 | Methods of normalization | | | Week 12 | High-throughput primer and probe design | | | Week 13 | Web resources about primer and probe design | | | Week 14 | Real-time PCR using SYBR® Green | | | Week 15 | Quantification of gene modification | | | Week 16 | End-of-term exam | | | |
| 1 | M. tevfik Dorak, Real Time PCR, Advanced Methods, Taylor and Francis, 2006 | | | 2 | Öğretim elemanının ders notları | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | | 2 | 35 | | Presentation | 15 | | 1 | 15 | | End-of-term exam | 16 | | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 2 | 14 | 28 | | Arasınav için hazırlık | 3 | 8 | 24 | | Arasınav | 2 | 1 | 2 | | Kısa sınav | 2 | 14 | 28 | | Dönem sonu sınavı için hazırlık | 3 | 14 | 42 | | Dönem sonu sınavı | 2 | 1 | 2 | | Diğer 1 | 4 | 14 | 56 | | Total work load | | | 224 |
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