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GRADUATE INSTITUTE of HEALTH SCIENCES / DEPARTMENT of OBESITY, DIABETES and ATHEROSCLEROSIS METABOLISM
Doctorate
Course Catalog
https://www.ktu.edu.tr/sabeobezite
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SABE
GRADUATE INSTITUTE of HEALTH SCIENCES / DEPARTMENT of OBESITY, DIABETES and ATHEROSCLEROSIS METABOLISM / Doctorate
Katalog Ana Sayfa
  Katalog Ana Sayfa  KTÜ Ana Sayfa   Katalog Ana Sayfa
 
 
ODM6003Research Techniques in Metabolic Diseases3+2+0ECTS:7.5
Year / SemesterFall Semester
Level of CourseThird Cycle
Status Elective
DepartmentDEPARTMENT of OBESITY, DIABETES and ATHEROSCLEROSIS METABOLISM
Prerequisites and co-requisitesNone
Mode of DeliveryFace to face, Lab work , Practical
Contact Hours14 weeks - 3 hours of lectures and 2 hours of practicals per week
LecturerProf. Dr. Ahmet MENTEŞE
Co-LecturerProf. Dr. Ahmet ALVER Asist. Prof. Fulya BALABAN YÜCESAN
Language of instructionTurkish
Professional practise ( internship ) None
 
The aim of the course:
The aim of this lecture is to make the students to learn the molecular techniques used in research laboratuaries and put in practice their theorical knowledge.
 
Programme OutcomesCTPOTOA
Upon successful completion of the course, the students will be able to :
PO - 1 : Can isolate DNA and RNA from various biomaterials61,3,4
PO - 2 : Can monitorize sample of DNA and RNA with agarose gel electrophoresis.61,3,4
PO - 3 : Can increase sample of DNA with PCR.61,3,4
PO - 4 : Can perform expression analysis with RT-PCR61,3,4
PO - 5 : Can perform protein analysis with Western Blotting.1,3,4
PO - 6 : Can perform cell and protein analysis with flow cytometry.61,3,4
PO - 7 : can be created Obesity and Diabetes model in experimental animals.6,101,3,4
CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome

 
Contents of the Course
1. Introduction of molecular laboratory tools. 2. Protein isolation and protein determination methods. 3. Imaging of DNA and RNA samples by agarose gel electrophoresis 4. Immunological analysis methods. 5. Protein analysis by Western blotting technique. 6. Isolation of DNA and RNA. 7. Reproduction of DNA samples by PCR technique 8. Analysis of expression by RT-PCR technique 9. Cell and protein analysis by flow cytometry technique 10. Establishing Obesity model in experimental animals. 11. Establishing Diabetes Model in Experimental Animals.
 
Course Syllabus
 WeekSubjectRelated Notes / Files
 Week 1Introduction of molecular laboratory tools.
 Week 2Protein isolation and protein determination methods.
 Week 3Imaging of DNA and RNA samples by agarose gel electrophoresis
 Week 4 Immunological analysis methods.
 Week 5 Protein analysis by Western blotting technique.
 Week 6Isolation of DNA and RNA.
 Week 7Reproduction of DNA samples by PCR technique
 Week 8Analysis of expression by RT-PCR technique
 Week 9midterm
 Week 10Cell and protein analysis by flow cytometry technique
 Week 11 Establishing Obesity model in experimental animals.
 Week 12 Establishing Obesity model in experimental animals.
 Week 13Establishing Diabetes Model in Experimental Animals
 Week 14Establishing Diabetes Model in Experimental Animals
 Week 15Establishing Diabetes Model in Experimental Animals
 Week 16 Final
 
Textbook / Material
1Temizkan, G., Arda N. 2007; Moleküler Biyolojide Kullanılan Yöntemler, Nobel Tıp Kitabevi,ISBN:9789754205831
 
Recommended Reading
1Demirkan, E. 2018; Moleküler Biyolojinin Temelleri, Dora Basım-Yayın, ISBN: 978-975-2447-67-7, Bursa
 
Method of Assessment
Type of assessmentWeek NoDate

Duration (hours)Weight (%)
Mid-term exam 9 30
07/03
04/2019		 2 30
Homework/Assignment/Term-paper 16 20
01/05
06/2019		 2 30
End-of-term exam 16 20
01/05
06/2019		 2 40
 
Student Work Load and its Distribution
Type of workDuration (hours pw)

No of weeks / Number of activity

Hours in total per term
Yüz yüze eğitim 2 14 28
Sınıf dışı çalışma 4 14 56
Laboratuar çalışması 2 14 28
Arasınav için hazırlık 8 1 8
Arasınav 2 1 2
Uygulama 3 7 21
Ödev 8 1 8
Dönem sonu sınavı için hazırlık 10 1 10
Dönem sonu sınavı 2 1 2
Total work load163