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| MLAB2013 | Biochemical Techniques | 3+0+0 | ECTS:3 | | Year / Semester | Fall Semester | | Level of Course | Short Cycle | | Status | Elective | | Department | DEPARTMENT of CHEMISTRY and CHEMICAL PROCESS TECHNOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Dr. Öğr. Üyesi Fatma YAYLACI KARAHALİL | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Aims to gain the basic biochemical techniques. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | The collect of biological samples, knows the processes and storage conditions. | 1,2,3,4,5,6,7,8 | 1 | | LO - 2 : | Sample preparation techniques can be used to understand the biochemistry research. | 1,2,3,4,5,6,7 | 1 | | LO - 3 : | The target molecule from a sample pre-separation techniques for the separation of biochemical materials and selects knows. | 1,2,3,4,5,6,7,8 | 1 | | LO - 4 : | From a biological measurement of enzyme activity, knows the principles that should be considered. | 1,2,3,4,5,6,7,8 | 1 | | LO - 5 : | Separation and purification techniques may be utilized to further purification and decide them. | 1,2,3,4,5,6,7,8 | 1 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Basic biochemical concepts, processes for the preparation of animal and plant tissue cultures, Biological Buffers, Sample Preparation Techniques in Biochemistry, extraction and pre-separation techniques, homogenization / lysis, centrifugation, microfiltration, ultrafiltration, dialysis, precipitation techniques Techniques of Separation and purification of biomolecules such as adsorption, gel , ion exchange, affinity, gas, high performance liquid chromatography, electrophoresis applications; Enzymatic Analysis Methods subjects aimed. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Basic biochemical concepts | | | Week 2 | Process for the preparation of animal and plant tissue culture, Sample Preparation Techniques in Biochemistry | | | Week 3 | Biological Buffers | | | Week 4 | Extraction and FrontSeparation Techniques | | | Week 5 | Homogenization / Shredding, Centrifuge, Microfiltration, Ultrafiltration | | | Week 6 | Dialysis, Deposition Techniques | | | Week 7 | Separation and purification of biomolecules Techniques | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 14/11/2016 | 1 | 50 | | End-of-term exam | 16 | 09/01/2017 | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 1 | 16 | 16 | | Sınıf dışı çalışma | 2 | 16 | 32 | | Arasınav için hazırlık | 1 | 9 | 9 | | Arasınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 2 | 16 | 32 | | Dönem sonu sınavı | 1 | 1 | 1 | | Total work load | | | 91 |
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