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| MBG4034 | Protein Engineering | 2+0+0 | ECTS:4 | | Year / Semester | Spring Semester | | Level of Course | First Cycle | | Status | Elective | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 2 hours of lectures per week | | Lecturer | Dr. Öğr. Üyesi Uğur UZUNER | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To raise awareness about the important parameters of proteins in terms of structure, function and functionality, to provide basic information about the building blocks of proteins and the basis of protein folding, to provide information about different protein engineering approaches and to explain the importance of such approaches in terms of industrial enzyme biotechnology with examples. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | Learn about the basic and three-dimensional structure of proteins. | 1,2,5,7 | | | LO - 2 : | Gain competence on the factors that play a role in the stability of proteins. | 1,2,5,7 | | | LO - 3 : | Have sufficient knowledge about enzyme kinetics and the important factors behind enzyme activity. | 1,2,5,7 | | | LO - 4 : | Understands the importance of natural folding and dynamics of proteins in terms of enzyme activity. | 1,2,5,7 | | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Throughout this course, the structure of proteins, basic properties, primary, secondary, tertiary and fourth folded protein structures, code-codon separation, protein folding, types of biochemical bonds responsible for protein folding and their roles in protein stability, diseases caused by misfolding, protein dynamics, protein Purification methods, protein-ligand interactions will be covered. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Introduction to Proteins | | | Week 2 | Amino acids, primary structure of protein | | | Week 3 | Protein secondary, tertiary and quaternary structures | | | Week 4 | Peptides and peptide bonds and the three-dimensional structure of proteins, | | | Week 5 | Advanced investigation on protein structures | | | Week 6 | Protein folding and misfolding related diseases | | | Week 7 | Protein Dynamics | | | Week 8 | Mid Term | | | Week 9 | Protein purification methods, cramotographic methods | | | Week 10 | Enzyme kinetics | | | Week 11 | Mechanism of action and inhibition of enzymes | | | Week 12 | Regulation of Enzymes and Bioenergy | | | Week 13 | Protein Ligand interactions | | | Week 14 | Student Seminar | | | Week 15 | Student Seminar | | | Week 16 | Final Exam | | | |
| 1 | Pravin Kaumaya 2012. Protein Engineering. Intech ISBN 978-953-51-0037-9
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| 1 | Garrett et al. 2013. Biochemistry. The First Canadian Edition | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 1 | 27.04.2021 | 50 | 30 | | Presentation | 12 | 25.05.2022 | 50 | 20 | | End-of-term exam | 14 | 21.06.2022 | 50 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 14 | 28 | | Sınıf dışı çalışma | 2 | 2 | 4 | | Arasınav için hazırlık | 2 | 1 | 2 | | Arasınav | 2 | 1 | 2 | | Ödev | 2 | 2 | 4 | | Dönem sonu sınavı için hazırlık | 2 | 1 | 2 | | Dönem sonu sınavı | 2 | 1 | 2 | | Total work load | | | 44 |
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