|
|
| MBG4026 | Genetic Engineering and Rec. DNA Technology | 3+0+0 | ECTS:4 | | Year / Semester | Spring Semester | | Level of Course | First Cycle | | Status | Compulsory | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Prof. Dr. Kadriye İNAN BEKTAŞ | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To learn the basic techniques of gene manipulation and the tools of cloning practically. To learn about the vectors used in cloning, to choose the appropriate vector for the situation. To learn different gene cloning strategies such as genomic DNA libraries, cDNA libraries and RACE. To learn the methods used in site-targeted mutagenesis and protein engineering. Using different bacteria, yeast and fungi as a cloning tool. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | To learn advanced analysis methods used in recombinant DNA technology and how to interpret the results obtained using these methods. | 1,2,7,12 | | | LO - 2 : | Advanced learning of the basics and importance of recombinant DNA technology, which is mainly used in the field of biotechnology. | | | | LO - 3 : | Gaining the necessary theoretical knowledge for specialization in genetic engineering related fields. | | | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Fundamental techniques of gene manipulation, cutting and joining of DNA molecules, properties of plasmid and phage vectors, cloning in single-stranded DNA vectors, cosmids, phasmids and other advanced vectors, genomic DNA libraries, cDNA libraries, RACE, DNA sequencing techniques, region-targeted mutagenesis and protein engineering , the use of bacteria and yeasts in cloning. |
| |
| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Basic techniques of gene manipulation | | | Week 2 | Basic techniques of gene manipulation | | | Week 3 | Cutting and joining of DNA molecules | | | Week 4 | Properties of plasmid vectors | | | Week 5 | Properties of phage vectors | | | Week 6 | Cloning in single-stranded DNA vectors | | | Week 7 | Cosmids and phasmids | | | Week 8 | Other advanced vectors | | | Week 9 | Mid-term exam | | | Week 10 | Genomic DNA libraries and cDNA libraries | | | Week 11 | RACE in gene cloning | | | Week 12 | DNA sequencing techniques | | | Week 13 | Site-targeted mutagenesis | | | Week 14 | Protein engineering | | | Week 15 | Protein engineering | | | Week 16 | End of term exam | | | |
| 1 | Principles of Gene Manipulation and Genomics, 7th Edition, Primrose, Twyman, Blackwell Publishing, ISBN: 978-1-4051-3544-3, 2006 | | | 2 | Gene Cloning and DNA Analysis: An Introduction, 6th Edition, T. Brown, Wiley-Blackwell, ISBN: 978-1-4051-8173-0, 2010 | | | |
| 1 | From Genes to Genomes: Concepts and Applicaitons of DNA Technology, 2nd Edition, Dale, von Schantz, John Wiley & Sons, Ltd, ISBN: 978-0-470-01734-0, 2007 | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | | 2 | 30 | | Presentation | 13
14
15 | | | 20 | | End-of-term exam | 16 | | | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 2 | 6 | 12 | | Arasınav için hazırlık | 3 | 8 | 24 | | Arasınav | 2 | 1 | 2 | | Dönem sonu sınavı için hazırlık | 3 | 6 | 18 | | Dönem sonu sınavı | 2 | 1 | 2 | | Total work load | | | 100 |
|