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| MBG3032 | Molecular Biology Laboratory | 0+0+2 | ECTS:2 | | Year / Semester | Spring Semester | | Level of Course | First Cycle | | Status | Compulsory | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 2 hours of laboratory per week | | Lecturer | Prof. Dr. Kadriye İNAN BEKTAŞ | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To provide students with general information about basic molecular techniques. Each lesson is dedicated to the cloning and isolation of nucleic acids and the identification of proteins. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | Has the ability to isolate DNA and RNA from eukaryotic and prokaryotic organisms | 4,7 | 1, | | LO - 2 : | Has the ability to amplify DNA by PCR | 4,7 | 1, | | LO - 3 : | Has the ability to display nucleic acids | 4,7 | 1, | | LO - 4 : | Has the ability to measure gene expression levels | 4,7 | 1, | | LO - 5 : | Has the ability to isolation proteins and determine quantity | 4,7 | 1, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Theoretical and practical teaching of current techniques in molecular biology. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Genomic DNA isolation from prokaryotic organisms | | | Week 2 | Plasmide DNA isolation from prokaryotic organisms | | | Week 3 | DNA isolation from eukaryotic organisms | | | Week 4 | Agarose gel electrophoresis | | | Week 5 | PCR | | | Week 6 | Ligation | | | Week 7 | Preparation of Competent cell | | | Week 8 | Transformation | | | Week 9 | Mid-term exam | | | Week 10 | Cloning | | | Week 11 | Protein immunoblottng (western) | | | Week 12 | Protein isolation | | | Week 13 | Protein quantitation | | | Week 14 | SDS-PAGE | | | Week 15 | Protein expression | | | Week 16 | Final Exam | | | |
| 1 | Öğretim elemanının ders notları | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | | 1 | 50 | | End-of-term exam | 16 | | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 14 | 28 | | Sınıf dışı çalışma | 0 | 0 | 0 | | Arasınav için hazırlık | 1 | 8 | 8 | | Arasınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 1 | 6 | 6 | | Dönem sonu sınavı | 1 | 1 | 1 | | Total work load | | | 44 |
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