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| MBG3030 | Molecular Biology | 4+0+0 | ECTS:6 | | Year / Semester | Spring Semester | | Level of Course | First Cycle | | Status | Compulsory | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 4 hours of lectures per week | | Lecturer | Prof. Dr. Kadriye İNAN BEKTAŞ | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To provide detailed knowledge about genome and operon structures and mechanisms of operation in bacteria. To provide information about transcriptional regulation mechanisms and transposons in prokaryotes. To have knowledge about mutations in prokayotic organisms and to distinguish mutation types. To distinguish between transformation, conjugation and transduction mechanisms and factors. In addition, to ensure that pupils are aware of experimental animals and genetic screening methods used to investigate eukaryotic phenotypes. To emphasize that basic genetic concepts such as replication, transcription and translation are more complex in eukaryotes. To enable students to use experimental approaches related to replication, transcription and translation for research purposes. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | Understand the structure of prokaryotes and eukaryotes genome; can reveal the differences | 1,3,7,11 | 1,5, | | LO - 2 : | Understand transcriptional regulation in prokaryotes and eukaryotes | 1,3,7,11 | 1,5, | | LO - 3 : | Learn plasmids, bacteriophages, transformation, cloning | 1,3,7,11 | 1,5, | | LO - 4 : | Understand DNA replication and mutation | 1,3,7,11 | 1,5, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Bacterial Genome and Operon Structure; Transcriptional Regulation; Lac Operon, Trp Operon; Mutations, Reversing and Suppression; Plasmids, Transformation, Conjugation; Bacteriophages, Transduction; Genomes in Eukaryotes, Model Organisms: Fly, Mouse and Arabidopsis Gene Identification and Function; DNA, RNA and Protein Manipulations; Eukaryotic DNA Replication and DNA repair; Presentation of gene control and transcription Chromatin structure; DNA repair mechanisms; Stability of mRNA and non-coding RNAs; Post Translational Modifications and Protein Degradation; Epigenetic changes in genome |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Course Description, Bacterial Genome and Operon Structure | | | Week 2 | Transcriptional Regulation | | | Week 3 | Lac Operon, Trp Operon | | | Week 4 | Mutations, Reversing and Suppression | | | Week 5 | Plasmids, Transformation, Conjugation | | | Week 6 | Bacteriophages, Transduction | | | Week 7 | Genomic structure in eukaryotes, Model organisms: Identification of gene and function of fly, mouse and Arabidopsis | | | Week 8 | DNA, RNA and Protein Manipulations | | | Week 9 | Mid-term exam | | | Week 10 | Eukaryotic DNA Replication and DNA repair | | | Week 11 | Introducing Gene Control and Transcription Chromatin Structure | | | Week 12 | DNA repair mechanisms | | | Week 13 | Stability of mRNA and non-coding RNAs | | | Week 14 | Post Translational Modifications and Protein Degradation | | | Week 15 | Epigenetic changes in genome | | | Week 16 | Final Exam | | | |
| 1 | Mol. Biol. Cell, edited by Alberts et al., 4th edition | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | | 1 | 40 | | Presentation | 13
14
15 | | 1 | 10 | | End-of-term exam | 16 | | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 4 | 14 | 56 | | Sınıf dışı çalışma | 3 | 6 | 18 | | Laboratuar çalışması | 0 | 0 | 0 | | Arasınav için hazırlık | 4 | 8 | 32 | | Arasınav | 2 | 1 | 2 | | Uygulama | 0 | 0 | 0 | | Klinik Uygulama | 0 | 0 | 0 | | Ödev | 0 | 0 | 0 | | Proje | 0 | 0 | 0 | | Kısa sınav | 0 | 0 | 0 | | Dönem sonu sınavı için hazırlık | 4 | 14 | 56 | | Dönem sonu sınavı | 2 | 1 | 2 | | Diğer 1 | 2 | 6 | 12 | | Diğer 2 | 0 | 0 | 0 | | Total work load | | | 178 |
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