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| MBG2008 | Physiology Laboratory | 0+0+2 | ECTS:3 | | Year / Semester | Spring Semester | | Level of Course | First Cycle | | Status | Compulsory | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 2 hours of laboratory per week | | Lecturer | Prof. Dr. Aykut SAĞLAM | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To teach students the structure and function of plant systems and their relations with each other, to provide basic experimental work on physiological events, to demonstrate the metabolic and growth events occurring in plants experimentally |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | Be able to explain the functions of plant systems together with their specific structures at the molecular, biochemical and physiological level | 4,5,8,11 | 1, | | LO - 2 : | To be able to conduct basic experimental studies on physiological phenomena in plant systems | 4,5,8,11 | 1, | | LO - 3 : | Experimental knowledge on chemical content of plants, plant-water relationship and mineral matter transport | 4,5,8,11 | 1, | | LO - 4 : | Observation-based information on water loss events (transpiration) in plants | 4,5,8,11 | 1, | | LO - 5 : | Obtain detailed experimental and observational information about photosynthesis, respiration and fermentation metabolisms | 4,5,8,11 | 1, | | LO - 6 : | Plant growth, development and hormonal relationship of these events can be experimentally understood | 4,11 | 1, | | LO - 7 : | learns to use computer-based software and develops level of foreign language | 4,5,8,11 | 1, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Experiments on qualitative and quantitative determination of some substances in plants, determination of osmotic potentials of plant tissues, permeability, transpiration, photosynthesis, respiration and fermentation experiments, effects of plant hormones on growth and development. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Qualitative and quantitative determination of certain substances in plants | | | Week 2 | Determination of osmotic potentials of plant tissues | | | Week 3 | Measurement of membrane permeability | | | Week 4 | Transport of water and water-soluble substances in plants and observation of transpiration | | | Week 5 | Extraction of photosynthetic pigments and determination of chlorophyllies, | | | Week 6 | separation of photosynthetic pigments by chromatography and determination of starch | | | Week 7 | Observation of respiration and fermentation events | | | Week 8 | Determination of ascorbic acid content | | | Week 9 | Midterm Exam | | | Week 10 | Observation of the effect of plant growth regulators on growth | | | Week 11 | Investigation of the selective herbicide effect of 2,4-D | | | Week 12 | Observation of epinasty event in tomato petioles | | | Week 13 | Effects of light, auxin and ethylene on hypocotyl hook opening | | | Week 14 | DNA isolation and imaging in plants | | | Week 15 | viability test by Tetrazolium test | | | Week 16 | Final Exam | | | |
| 1 | Kadıoğlu, A., Sağlam, A., Avcı, U. 2003; Bitki Fizyolojisi Laboratuar Kılavuzu, Eser Ofset, Trabzon. | | | |
| 1 | Taiz, L and Zeiger, E. 2007;Bitki Fizyolojisi (Çeviri editörü: İsmail TÜRKAN), Palme yayıncılık, Ankara. | | | 2 | Kadioglu, A, 2007; Bitki Fizyolojisi, Esen Ofset, Trabzon. | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | | 2 | 50 | | End-of-term exam | 16 | | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 14 | 28 | | Sınıf dışı çalışma | 1 | 14 | 14 | | Laboratuar çalışması | 2 | 14 | 28 | | Arasınav için hazırlık | 1 | 8 | 8 | | Arasınav | 2 | 1 | 2 | | Uygulama | 0 | 0 | 0 | | Klinik Uygulama | 0 | 0 | 0 | | Ödev | 0 | 0 | 0 | | Proje | 0 | 0 | 0 | | Kısa sınav | 0 | 0 | 0 | | Dönem sonu sınavı için hazırlık | 1 | 14 | 14 | | Dönem sonu sınavı | 2 | 1 | 2 | | Diğer 1 | 0 | 0 | 0 | | Diğer 2 | 0 | 0 | 0 | | Total work load | | | 96 |
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