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| MBG2000 | Molecular Microbiology | 3+0+0 | ECTS:5 | | Year / Semester | Spring Semester | | Level of Course | First Cycle | | Status | Compulsory | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Doç. Dr. Hacer MURATOĞLU | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To show the technological methods related to molecular diagnostic tests and the points that technological tests can reach. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | Learns the principles of microbial diagnosis | 1,2,3,7 | 1, | | LO - 2 : | Have knowledge about microbial diagnostic applications | 1,2,3,7 | 1, | | LO - 3 : | Determines which diagnostic method to choose according to the problem | 1,2,3,7 | 1, | | LO - 4 : | Analyzes the data obtained as a result of the application of the methods | 1,2,3,7 | 1, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Culture Verification and Direct Detection Probes, Nucleic Acid Amplification Methods, Diagnostic Sequence Analysis and Mutation Detection, Molecular Typing Methods, Detection Methods, Detection and Identification of Bacterial Pathogens, Identification and Identification of Viruses. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Culture Verification and Direct Detection Probes | | | Week 2 | Nucleic Acid Replication Methods | | | Week 3 | Diagnostic Sequence Analysis and Mutation Detection-Mutation Scan | | | Week 4 | Diagnostic Sequence Analysis and Mutation Detection-Mutation Detection: Approaches Based on PCR | | | Week 5 | Molecular Typing Methods-Pulsed-Field Gel Electrophoresis-PCR Amplification Based Microbial Typing | | | Week 6 | Molecular Typing Methods-Pulsed-Field Gel Electrophoresis-PCR Amplification Based Microbial Typing | | | Week 7 | Detection Methods-Detection of Amplification Products | | | Week 8 | Detection Methods-Gel Electrophoresis, Southern Hybridization, and Restriction Fragment Length Polymorphism | | | Week 9 | Midterm | | | Week 10 | Detection Methods-Microplate Detection Systems for Detection and Identification of Amplification Products | | | Week 11 | Detection and Identification of Bacterial Pathogens-16S Ribosomal DNA Sequence Analysis for Identification of Bacteria in the Clinical Microbiology Laboratory | | | Week 12 | Detection and Identification of Bacterial Pathogens-Identification of Antimicrobial Resistance Genes of Microorganisms and Mutations Associated with Antimicrobial Resistance | | | Week 13 | Detection and Identification of Bacterial Pathogens-Application of Genomic Knowledge in Diagnosis, Treatment and Control of Bacterial Infections | | | Week 14 | Detection and Identification of Viruses-DNA Viruses | | | Week 15 | Detection and Identification of Viruses-RNA Viruses | | | Week 16 | Final Exam | | | |
| 1 | Persing H. D. (Editör), Tekeli ve Ustaçelebi (Çeviri Editörleri) 2006; Moleküler Mikrobiyoloji, Palme Yayıncılık, ISBN 9944-341-13-4, Ankara. | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 30.03.2024 | 1 | 50 | | End-of-term exam | 16 | 01.07.2024 | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 15 | 45 | | Sınıf dışı çalışma | 3 | 15 | 45 | | Arasınav için hazırlık | 2 | 12 | 24 | | Arasınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 2 | 16 | 32 | | Dönem sonu sınavı | 2 | 1 | 2 | | Total work load | | | 149 |
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