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| KIM3021 | Biochemistry Laboratory | 0+0+2 | ECTS:3 | | Year / Semester | Fall Semester | | Level of Course | First Cycle | | Status | Compulsory | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 2 hours of laboratory per week | | Lecturer | Prof. Dr. Sevgi KOLAYLI | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Introducing some techniques used to understand basic concepts in isolation, purification and characterization of biomolecules, and their structure-function relationship, determination and quantitation of biomolecules, Introducing Some Techniques Used To Understand Basic Concepts and Methodology in Metabolism, Biomolecule ? Ligand İnteractions and Biomolecule Functions, and determine biological activity |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | will be able to perform the techniques for the determination and identification of biomolecules | 1,2,4,7 | 1,2,4, | | LO - 2 : | will be able to determine the techniques for isolation, purification and characterization of biomolecules | 1,2,4,7 | 1,2,4, | | LO - 3 : | will learn how metabolism can be monitored | 1,2,4,7 | 1,2,4, | | LO - 4 : | will learn how to investigate molecular interactions. | 1,2,4,7 | 1,2,4, | | LO - 5 : | will prepare for clinical and industrial laboratory
applications | 1,2,4,7 | 1,2,4, | | LO - 6 : | will be able to perform the techniques how the biological active molecules determine | 1,2,4,7 | 1,2,4, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| General concepts in use of biochemical techniques and analysis of data, Isolation, purification and characterization of biomolecules, Determination and quantitation of biomolecules, Investigation of biomolecular structure-function relationship, Investigation of biomolecule-ligand interactions, Monitoring of metabolic pathway, Investigation of enzyme kinetics, Determination of biological activity |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Laboratory introduction, rules, acquaintance | | | Week 2 | Recognition reactions of proteins | | | Week 3 | Recognition reactions of sugars | | | Week 4 | Buffurs | | | Week 5 | Enzymatic reaction 1 | | | Week 6 | Enzymatic reaction 2 | | | Week 7 | Biuret recations | | | Week 8 | Spectrophotometric determination of cholesterol | | | Week 9 | Purification lesitin rom egg | | | Week 10 | Determinatio of antioxidant activiity | | | Week 11 | Protein solubility-2 | | | Week 12 | Protein solubility-2 | | | Week 13 | | | | Week 14 | | | | Week 15 | | | | Week 16 | | | | |
| 1 | M. Küçük (Editör, 2. Ed.), 2011, Temel Biyokimya Laboratuarı, Derya Kitabevi, Trabzon | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 10/05/20022 | 2 | 50 | | In-term studies (second mid-term exam) | 16 | 15/01/2023 | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 11 | 22 | | Sınıf dışı çalışma | 1 | 11 | 11 | | Arasınav için hazırlık | 8 | 1 | 8 | | Arasınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 15 | 1 | 15 | | Total work load | | | 57 |
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