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| MBG3027 | Tissue Culture | 3+0+0 | ECTS:5 | | Year / Semester | Fall Semester | | Level of Course | First Cycle | | Status | Compulsory | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Dr. Öğr. Üyesi Tuğba TOPAL | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | It is aimed for students to have information about the cell culture development process, to comprehend the primary and secondary cell culture development steps, to learn laboratory equipment and rules, to obtain information about laboratory safety, to obtain information about current culture techniques. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | Knows primary cell culture development steps | 1,3,7 | 1, | | LO - 2 : | Knows how to access detailed information about a cell line | 1,3,7 | 1,3, | | LO - 3 : | Knows laboratory safety and rules. | 1,3,7 | 1, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Historical development of cell culture, cell culture development steps, cell culture laboratory rules and layout, Cell culture laboratory equipment and materials, Contamination and aseptic techniques, Laboratory safety, Culture media components, substrate - gas phase - medium - heat, Sterilization and other preparations, Degradation of tissues and development of primary culture, Characterization of culture cells, 3D cultures, lab-on-a-chip / human-on-a-chip applications, Plant tissue culture |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Historical development of cell culture | | | Week 2 | Cell culture development steps | | | Week 3 | Cell culture development steps | | | Week 4 | Cell culture laboratory rules and organization | | | Week 5 | Cell culture laboratory equipment and supplies | | | Week 6 | Cell culture laboratory equipment and supplies | | | Week 7 | Contamination and aseptic techniques | | | Week 8 | Mid-term exam | | | Week 9 | Laboratory safety | | | Week 10 | Culture media components | | | Week 11 | Culture media components | | | Week 12 | Tissue lysis and primary culture development | | | Week 13 | Characterization of cultured cells | | | Week 14 | 3D cultures, lab-on-a-chip / human-on-a-chip applications | | | Week 15 | Plant tissue culture | | | Week 16 | Final Exam | | | |
| 1 | Demir İ, Hücre Kültürü Ders Notları, Karadeniz Teknik Üniversitesi Biyoloji Bölümü | | | |
| 1 | Freshney, I.A., 2010; Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Wiley-Blackwell | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 9. hafta | 1 | 30 | | In-term studies (second mid-term exam) | 14 | 14. hafta | 1 | 20 | | End-of-term exam | 16 | 16. hafta | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 1 | 15 | 15 | | Arasınav için hazırlık | 1.5 | 7 | 10.5 | | Arasınav | 1 | 1 | 1 | | Ödev | 1 | 10 | 10 | | Dönem sonu sınavı için hazırlık | 1.5 | 15 | 22.5 | | Dönem sonu sınavı | 1 | 1 | 1 | | Total work load | | | 102 |
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