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| MBG3025 | Molecular Genetics Laboratory | 0+0+2 | ECTS:3 | | Year / Semester | Fall Semester | | Level of Course | First Cycle | | Status | Compulsory | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 2 hours of laboratory per week | | Lecturer | Doç. Dr. Selcen ÇELİK UZUNER | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | This course aims to teach students basic molecular techniques and reinforce what they learn in Molecular Biology and Genetics by using modern methods |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | Must be able to treat and isolate nucleic acids carefully in the laboratory | 2,4,6 | 4 | | LO - 2 : | Know the principles of nucleic acid analysis methods | 2,4,6 | 4 | | LO - 3 : | Basics the safety rules genetic laboratory | 2,4,6 | 4 | | LO - 4 : | Develop critical thinking, scientific reasoning, quantitative reasoning skills in the design, analysis and interpretation of genetic research | 2,4,6 | 3,4 | | LO - 5 : | Be able to understand and explain the use of modern molecular biology techniques | 2,4,6 | 3,4 | | LO - 6 : | Transfer laboratory results in written and verbal form effectively and efficiently | 2,4,6 | 4 | | LO - 7 : | Apply basic molecular biology laboratory knowledge to investigate biomolecules, cellular processes and diseases | 2,4,6 | 5 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Karyotyping, Mitosis/Meiosis, Animal tissues, Making pedigrees, DNA isolation, PCR, QPCR and agarose gel electrophoresis, monohibit and dihybrid crossing with Drosophila, Mitochondria and nucleus fluorescence staining in culturing cells
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Karyotyping - Chromosomal Banding Methods | | | Week 2 | Karyotyping - Chromosomal Banding Methods | | | Week 3 | Karyotyping - Chromosomal Banding Methods | | | Week 4 | Mitosis and Meiosis | | | Week 5 | Tissues in animals | | | Week 6 | Obervation of Barr Body | | | Week 7 | Making a Pedigree - A practice for genetic counseling | | | Week 8 | Making a Pedigree - A practice for genetic counseling | | | Week 9 | Midterm exam | | | Week 10 | RNA isolation and QPCR (gene expression and/or copynumber variation) | | | Week 11 | RNA isolation and QPCR (gene expression and/or copynumber variation) | | | Week 12 | Gene knock-out | | | Week 13 | Polytene polychromosomes in Drosophila | | | Week 14 | Monohibit and dihybrid crossing with Drosophila and statistical analysis of results | | | Week 15 | Analysis for DNA fingerprinting | | | Week 16 | Final Exam | | | |
| 1 | Thomas L Mertens and Robert L. Hammersmith, Genetics Laboratory Investigations (13th Edition), Printice Hall, New Jersey, USA, 2006, ISBN: 0-15-019330-5 | | | 2 | Watson, James D., Tania A. Baker, Stephen P. Bell, and Alexander Gann (2007), Molecular Biology of the Gene. Sixth Edition. San Francisco, CA: Benjamin Cummings | | | |
| 1 | Molecular Biology Laboratory Notes, Belduz vd. 2008, Trabzon | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | | 1 | 30 | | End-of-term exam | 16 | | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 16 | 32 | | Sınıf dışı çalışma | 2 | 14 | 28 | | Laboratuar çalışması | 2 | 14 | 28 | | Arasınav için hazırlık | 2 | 8 | 16 | | Arasınav | 1 | 1 | 1 | | Uygulama | 2 | 16 | 32 | | Kısa sınav | 1 | 10 | 10 | | Dönem sonu sınavı için hazırlık | 2 | 5 | 10 | | Dönem sonu sınavı | 1 | 1 | 1 | | Total work load | | | 158 |
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