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| MBG2003 | Cell Biology Laboratory | 0+0+2 | ECTS:3 | | Year / Semester | Fall Semester | | Level of Course | First Cycle | | Status | Compulsory | | Department | DEPARTMENT of MOLECULAR BIOLOGY and GENETICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 2 hours of laboratory per week | | Lecturer | Prof. Dr. Kadriye İNAN BEKTAŞ | | Co-Lecturer | No | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | The aim of this course is to give basic concepts regarding the structure, functions and physiology of the cell and to ensure an understanding of these concepts and subjects. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | They will have detailed knowledge of the various properties of the cells | 4,7 | 1,4, | | LO - 2 : | They will have experience in cell biology laboratory techniques. | 4,7 | 1,4, | | LO - 3 : | They will take part in discussions with their friends on cell biology laboratory experiences. | 4,7 | 1,4, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Cell biology laboratory environments and rules; Use of the microscope and cell diversity, Microscopic measurement units and measurement under the microscope; Preparation of pH and biological buffers; Genomic and Plasmid DNA isolation and analysis, Protein isolation and analysis, TLC |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Cell biology laboratory conditions and rules | | | Week 2 | Microscopic use and cell diversity | | | Week 3 | Microscopic measurement units and measurement at microscope | | | Week 4 | pH and preparation of biological buffers | | | Week 5 | Isolation of nucleic acids | | | Week 6 | Analysis of nucleic acids | | | Week 7 | Isolation of protein | | | Week 8 | Analysis of protein | | | Week 9 | Mid-term exam | | | Week 10 | Cell culture technique; | | | Week 11 | Enzymes and enzyme activity | | | Week 12 | Homework delivery | | | Week 13 | Cell division and chromosome morphology | | | Week 14 | Substance transport to cells | | | Week 15 | TLC | | | Week 16 | End-of-term exam | | | |
| 1 | Heidcamp, W. H. 1995. Cell Biology Laboratory Manual. Saint Peter, Minnesota, USA | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | | 1 | 40 | | Homework/Assignment/Term-paper | 12 | | 2 | 10 | | End-of-term exam | 16 | | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 14 | 28 | | Sınıf dışı çalışma | 2 | 6 | 12 | | Arasınav için hazırlık | 2 | 8 | 16 | | Arasınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 2 | 14 | 28 | | Dönem sonu sınavı | 1 | 1 | 1 | | Total work load | | | 86 |
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