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| MBIO1004 | Basic Techniques in Biochemistry | 3+0+2 | ECTS:6 | | Year / Semester | Spring Semester | | Level of Course | Short Cycle | | Status | Compulsory | | Department | DEPARTMENT of CHEMISTRY and CHEMICAL PROCESS TECHNOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures and 2 hours of laboratory per week | | Lecturer | Doç. Dr. Elif AYAZOĞLU DEMİR | | Co-Lecturer | Prof. Dr. Melike Yıldırım Akatın | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Basic biochemical techniques to gain the theoretical and practical competence. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | gain basic knowledge of chemistry and biochemistry . | 10,11,12,22 | 1,4, | | LO - 2 : | acquire basic skills such as preparation of buffers , pH measurement, homogenizing ,making centrifuges. | 10,11,12,24 | 1,4, | | LO - 3 : | implement laboratory procedures such as spectrophotometry, HPLC , SDS -PAGE. | 10,11,12,24 | 1,4, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| The importance of water in biochemistry , water types and purification techniques for biochemical assays , sample preparation techniques in biochemistry, biological buffers, cell disruption and homogenization, centrifugation, extraction and pre-separation techniques, chromatographic methods, electrophoretic methods, polymerase chain reaction (PCR) , immunological techniques , HPLC, spectroscopic methods in biochemistry |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | The importance of water in biochemistry, laboratory preparation, principles of weighing, SI units | | | Week 2 | The solution preparation (concentration calculation) | | | Week 3 | The solution preparation (concentration calculation) | | | Week 4 | pH measurement and buffer preparation | | | Week 5 | Extraction | | | Week 6 | Homogenization techniques | | | Week 7 | Homogenization techniques | | | Week 8 | Centrifugation | | | Week 9 | Midterm exam | | | Week 10 | The basic principles of chromatography , chromatography types and applications | | | Week 11 | The basic principles of chromatography , chromatography types and applications | | | Week 12 | Basic principle of electrophoresis, types and applications of electrophoresis, buffer preparation | | | Week 13 | Polymerase chain reaction (PCR) | | | Week 14 | Immunological techniques , RIA, ELISA | | | Week 15 | Spectroscopic methods in biochemistry | | | Week 16 | Final exam | | | |
| 1 | Telefoncu, A., 2000, Biyokimyada Temel ve Modern Teknikler, İzmir. | | | 2 | Telefoncu, A., 2007, Biyokimyada ve Moleküler Biyolojide Modern Teknikler, İzmir | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 03.05.2024 | 1 | 30 | | Laboratory exam | 1
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15 | | 0,30 | 20 | | End-of-term exam | 16 | 29.06.2024 | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 2 | 14 | 28 | | Laboratuar çalışması | 2 | 14 | 28 | | Arasınav için hazırlık | 2 | 8 | 16 | | Arasınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 6 | 6 | 36 | | Dönem sonu sınavı | 1 | 1 | 1 | | Diğer 1 | 12 | 2 | 24 | | Total work load | | | 176 |
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