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| MBIO2017 | cell and tissue culture preparation technics | 3+0+0 | ECTS:4 | | Year / Semester | Fall Semester | | Level of Course | Short Cycle | | Status | Elective | | Department | DEPARTMENT of CHEMISTRY and CHEMICAL PROCESS TECHNOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Doç. Dr. Elif AYAZOĞLU DEMİR | | Co-Lecturer | Dr. Elif AYAZOĞLU DEMİR | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Cell and tissue culture preparation techniques to gain theoretical and practical competence |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | Gain basic cell knowledge. | 10,14,20 | 1, | | LO - 2 : | Describe the biological properties of primer tissue cells and culture cells. | 9,14,20 | 1, | | LO - 3 : | Learn basic knowledge about proliferation, transformation, cell death, differentiation processes. | 9,14,20 | 1, | | LO - 4 : | Know the equipment, consumables and media used in cell culture techniques. | 9,14,20 | 1, | | LO - 5 : | Know biosafety rules applied in cell culture laboratory, sterilization and aseptic techniques. | 9,14,20 | 1, | | LO - 6 : | It performs media preparation, suspension and monolayer culturing, seeding, termination, cell freezing / thawing and simple viability tests. | 9,14,20 | 1, | | LO - 7 : | Describe cell characterization and discrimination techniques. | 9,14,20 | 1, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Cell Related Definitions and Theories, Basic Cellular Diversity and Function of the General Classification of Living Cell Morphology and Cytology, Cell Metabolism, Cell Growth and Life Factors, metamorphosis and mutation, cellular Transfer Phenomena, phage and cellular Importance of Cellular Stress Factors, Aseptic Technique and Good Cell Culture Practice Techniques (GCCP), Cell transfer Operations in aseptic conditions, Enumeration techniques, direct - indirect cell staining and microscopic examination techniques, Gram and capsules Painting Techniques, Endospor Painting and bacterial Mobility Study Techniques, Biochemical Identification of test with bacterial Direct serological methods, indirect serological methods |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | General information about cell and tissue culture | | | Week 2 | Fundamental Structures and Cellular Functions | | | Week 3 | Control of Cell Cycle and Apoptosis | | | Week 4 | Cell Morphology and Cytology, Cell Metabolism, Cell Growth Factors and Life | | | Week 5 | Metamorphosis and mutation, cellular Transfer Phenomena, Fajr and Importance of Cellular, Cellular Stress Factors | | | Week 6 | Aseptic Technique Practices and Good Cell Culture Techniques (GCCP), Good Manufacturing Practice (GMP) | | | Week 7 | Aseptic Conditions in Cell Transfer Operations | | | Week 8 | Enumeration techniques, direct - indirect cell staining and microscopic examination techniques | | | Week 9 | Midterm | | | Week 10 | Enumeration techniques, direct - indirect cell staining and microscopic examination techniques | | | Week 11 | Cytotoxicity tests. | | | Week 12 | Identification of Bacteria | | | Week 13 | Direct Serological Methods | | | Week 14 | Indirect Serological Methods | | | Week 15 | An overview | | | Week 16 | Final exam | | | |
| 1 | Topal, Ş., 2004, Hücre Kültür Teknikleri-I, İstanbul. | | | |
| 1 | Telefoncu, A., 2007, Biyokimyada ve Moleküler Biyolojide Modern Teknikler, İzmir | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 01.12.2023 | 1 | 50 | | End-of-term exam | 16 | 24.01.2024 | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 2 | 14 | 28 | | Arasınav için hazırlık | 2 | 8 | 16 | | Arasınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 2 | 13 | 26 | | Dönem sonu sınavı | 1 | 1 | 1 | | Diğer 1 | 6 | 1 | 6 | | Total work load | | | 120 |
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