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| SECB484 | MODERN TECHNICS IN BIOCHEMISTRY | 2+0+0 | ECTS:3 | | Year / Semester | Spring Semester | | Level of Course | First Cycle | | Status | Elective | | Department | DEPARTMENT of CHEMISTRY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 2 hours of lectures per week | | Lecturer | -- | | Co-Lecturer | Lecturers in Biochemistry Division | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Understanding of modern biochemical technics. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | learn the usage field of technics used in biochemistry and learn usage aim of them | 1,6 | 1 | | LO - 2 : | understand the importance of internet for biochemistry and learn the usage of beneficial internet sites | 1,6 | 1 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Introduction to technics used in biochemistry, Electrophoretic Technics (Protein electrophoresis, DNA electrophoresis, 2D electrophoresis, Blotting technics), Recombinant DNA Technics (Isolation and analysis of nucleic acids, PCR, Construction and investigation of genomic DNA libraries, Gene cloning, Transformation, Gen mutation), Protein analysis Technics (Technics used in isolation, purification and characterization of proteins, Proteomics (Amino acid sequencing and Activity tests of enzymes)), Biochemistry and internet (Usage of internet in biochemistry (Animation sites of metabolic pathways and its application), Gen and genomics data-bases). |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Introduction to technics used in biochemistry, | | | Week 2 | Electrophoretic Technics (Protein electrophoresis, DNA electrophoresis, 2D electrophoresis, Blotting technics), | | | Week 3 | Electrophoretic Technics (Protein electrophoresis, DNA electrophoresis, 2D electrophoresis, Blotting technics), | | | Week 4 | Recombinant DNA Technics (Isolation and analysis of nucleic acids, PCR, Construction and investigation of genomic DNA libraries, Gene cloning, Transformation, Gen mutation), | | | Week 5 | Recombinant DNA Technics (Isolation and analysis of nucleic acids, PCR, Construction and investigation of genomic DNA libraries, Gene cloning, Transformation, Gen mutation), | | | Week 6 | Recombinant DNA Technics (Isolation and analysis of nucleic acids, PCR, Construction and investigation of genomic DNA libraries, Gene cloning, Transformation, Gen mutation), | | | Week 7 | Recombinant DNA Technics (Isolation and analysis of nucleic acids, PCR, Construction and investigation of genomic DNA libraries, Gene cloning, Transformation, Gen mutation), | | | Week 8 | Recombinant DNA Technics (Isolation and analysis of nucleic acids, PCR, Construction and investigation of genomic DNA libraries, Gene cloning, Transformation, Gen mutation), | | | Week 9 | Mid-term exam | | | Week 11 | Protein analysis Technics (Technics used in isolation, purification and characterization of proteins, | | | Week 12 | Proteomics (Amino acid sequencing and Activity tests of enzymes) | | | Week 13 | Proteomics (Amino acid sequencing and Activity tests of enzymes)), | | | Week 14 | Biochemistry and internet (Usage of internet in biochemistry (Animation sites of metabolic pathways and its application), Gen and genomics data-bases). | | | Week 15 | Biochemistry and internet (Usage of internet in biochemistry (Animation sites of metabolic pathways and its application), Gen and genomics data-bases). | | | Week 16 | End-of-term exam | | | |
| 1 | Telefoncu, A. 2000, Biyokimyada Temel ve Modern Teknikler, Ege Ünv. Yayınları, İzmir | | | 2 | Wilson, K. and Walker, J. (Ed.) 2005, Principles and Techniques of Biochemistry and Molecular Biology, Cambridge Univ. Press, Sixth edition, Cambridge | | | |
| 1 | Telefoncu, A. 2003, Biyoinformatik-I, İzmir | | | 2 | Yıldırım, A., Bardakçı, F., Karataş, M. Ve Tanyolaç, B. 2007, Moleküler Biyoloji, Nobel Yayın Dağıtım, Ankara | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 07.04.2016 | 0,5 | 50 | | End-of-term exam | 16 | 27.05.2016 | 0,6 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 14 | 28 | | Sınıf dışı çalışma | 2 | 14 | 28 | | Arasınav için hazırlık | 9 | 1 | 9 | | Arasınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 9 | 1 | 9 | | Dönem sonu sınavı | 1 | 1 | 1 | | Total work load | | | 76 |
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