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GRADUATE INSTITUTE of NATURAL and APPLIED SCIENCES / DEPARTMENT of MOLECULAR BIOLOGY and GENETICS
MOLECULAR BIOLOGY and GENETICS Master Program
Course Catalog
http://www.ktu.edu.tr/molekulerb
Phone: +90 0462 377 3686
FBE
GRADUATE INSTITUTE of NATURAL and APPLIED SCIENCES / DEPARTMENT of MOLECULAR BIOLOGY and GENETICS / MOLECULAR BIOLOGY and GENETICS Master Program
Katalog Ana Sayfa
  Katalog Ana Sayfa  KTÜ Ana Sayfa   Katalog Ana Sayfa
 
 

MBGL5034Genetic and protein engineering3+0+0ECTS:7.5
Year / SemesterSpring Semester
Level of CourseSecond Cycle
Status Elective
DepartmentDEPARTMENT of MOLECULAR BIOLOGY and GENETICS
Prerequisites and co-requisitesNone
Mode of Delivery
Contact Hours14 weeks - 3 hours of lectures per week
LecturerDoç. Dr. Uğur UZUNER
Co-Lecturer
Language of instructionTurkish
Professional practise ( internship ) None
 
The aim of the course:
The aim of this course is to raise awareness of students about the latest developments in gene and protein engineering and current techniques. This approach also targets to contribute to the knowledge and development of students on agriculture, biotechnology, food&feed, health and medicine by unraveling the current practices and potentials.
 
Programme OutcomesCTPOTOA
Upon successful completion of the course, the students will be able to :
PO - 1 : Be aware of current applications in gene and protein engineering3,4,5,61,5
PO - 2 : Recognize the functional structures of the genes and acquire critical elements3,4,5,61,5
PO - 3 : Has knowledge about protein folding, functionality and engineering3,4,5,61,5
PO - 4 : Design complex expression cassettes adaptable to biotechnology3,4,5,61,5
CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome

 
Contents of the Course
This course will cover topics such as introduction to recombinant DNA technologies, prokaryotic and eukaryotic gene structures, isolation and electrophoresis of nucleic acids and proteins, Modern PCR applications, Design of recombinant gene expression cassettes (vectors and hosts), Different transformation techniques in homologus and heterologous gene expression, Protein folding and 3 dimensional structure modeling , Bioinformatics and modern approaches in protein engineering, techniques for determining protein-protein and protein-DNA interactions, CRISPR in gene engineering, VIGS and RNAi mechanisms and gene therapy applications.
 
Course Syllabus
 WeekSubjectRelated Notes / Files
 Week 1Introduction to recombinant DNA technologies
 Week 2Prokaryotic and eukaryotic gene structures
 Week 3Isolation and electrophoresis of nucleic acids and protein
 Week 4Modern PCR applications (qPCR, RTPCR, overlapping PCR, Gibson assembly)
 Week 5The design of recombinant gene expression cassettes (vectors and hosts)
 Week 6Different transformation techniques in homologus and heterologous gene expression
 Week 7Different approaches in gene and protein engineering
 Week 8Mid Term
 Week 9Protein folding and 3D structure modeling
 Week 10Bioinformatics and modern approaches in protein engineering
 Week 11Techniques for determining protein-protein and protein-DNA interactions
 Week 12Gene engineering of genomes by using CRISPR-cas9 system
 Week 13VIGS and RNAi mechanisms in gene engineering.
 Week 14Student Seminars
 Week 15Gene therapy applications
 Week 16Final Exam
 
Textbook / Material
1Molecular Biology of the Gene. 7th Edition., JAMES D. WATSON et al., 2014/ CSH Press/ISBN-13: 978-0-321-76243-6
 
Recommended Reading
1Protein Bioinformatics: From Sequence to Function. M. Michael Gromiha et al. 2010 / Elsevier, ISBN-13: 978-81-312-2297-3
 
Method of Assessment
Type of assessmentWeek NoDate

Duration (hours)Weight (%)
Mid-term exam 8 18.11.2021 3 30
Presentation 14 18.01.2022 3 20
End-of-term exam 16 25.01.2022 3 50
 
Student Work Load and its Distribution
Type of workDuration (hours pw)

No of weeks / Number of activity

Hours in total per term
Yüz yüze eğitim 3 14 42
Sınıf dışı çalışma 3 14 42
Arasınav için hazırlık 3 6 18
Arasınav 3 1 3
Uygulama 3 6 18
Ödev 3 6 18
Proje 3 6 18
Kısa sınav 3 6 18
Dönem sonu sınavı için hazırlık 3 6 18
Dönem sonu sınavı 3 1 3
Total work load198