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| TMIK542 | Immune Assays | 1+2+0 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of MEDICAL MICROBIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Lab work , Practical | | Contact Hours | 14 weeks - 1 hour of lectures and 2 hours of practicals per week | | Lecturer | -- | | Co-Lecturer | None | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Include teaching and practice of the in vitro antigen-antibody reactions |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | do and evaluate precipitation assays. | 1,2,3,4,5 | 1,5 | | PO - 2 : | do and evaluate agglutination assays. | 1,2,3,4,5 | 1,5 | | PO - 3 : | do and evaluate complement fixation assay. | 1,2,3,4,5 | 1,5 | | PO - 4 : | do and evaluate immunoflourescence tests (direct/indirect). | 1,2,3,4,5 | 1,5 | | PO - 5 : | do and evaluate ELISA tests. | 1,2,3,4,5 | 1,5 | | PO - 6 : | do and evaluate radioimmunoassay tests. | 1,2,3,4,5 | 1,5 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Precipitation, agglutination (co-agglutination, hemagglutination, hemagglutination inhibition, Coombs reaction) , complement fixation test, immuneflourescence tests (direct/indirect) , ELISA, radioimmunoassay, chromatographic technics, lymphocyte tests, flow cytometry, electrophoresis, antigen preperation techniques, skin tests. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Precipitation reactions | | | Week 2 | agglutination tests-1 (co-agglutination) | | | Week 3 | Agglutination tests-2 (hemagglutination, hemagglutination inhibition tests) | | | Week 4 | Agglutination tests (Coombs reactions) | | | Week 5 | complement fixation test | | | Week 6 | immunoflourescence tests (direct/indirect) | | | Week 7 | ELISA | | | Week 8 | Mid-term exam | | | Week 9 | Radioimmunoassay | | | Week 10 | Chromatographic technics | | | Week 11 | Lymphocyte tests | | | Week 12 | Student's presentation | | | Week 13 | Flow cytometry | | | Week 14 | Electrophoresis principles and applications | | | Week 16 | End-of-term exam | | | |
| 1 | Abbas, A.K. Lichtman, A.H. 2007; Temel İmmünoloji, İstanbul Tıp Kitabevi, İstanbul. | | | |
| 1 | Kılışturgay, K. 2003; İmmünoloji, Nobel ve Güneş Yayınevi, İstanbul. | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | | 1 | 30 | | Presentation | 12 | | 1 | 20 | | End-of-term exam | 17 | | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 1 | 2 | 2 | | Sınıf dışı çalışma | 2 | 8 | 16 | | Laboratuar çalışması | 2 | 16 | 32 | | Arasınav için hazırlık | 15 | 2 | 30 | | Arasınav | 1 | 1 | 1 | | Ödev | 4 | 2 | 8 | | Dönem sonu sınavı için hazırlık | 30 | 2 | 60 | | Dönem sonu sınavı | 1 | 1 | 1 | | Total work load | | | 150 |
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