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| TBI 625 | Recombinant DNA Technology | 2+0+2 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Third Cycle | | Status | Compulsory | | Department | DEPARTMENT of MEDICAL BIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Group study | | Contact Hours | 14 weeks - 2 hours of lectures and 2 hours of laboratory per week | | Lecturer | -- | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Aim of this course is; understanding the theory and applications of recombinant DNA technology by students. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Student defines biochemical reagents used in recombinant DNA technology. | | | | PO - 2 : | Student defines vectors used in recombinant DNA technology and can compare them. | | | | PO - 3 : | Student knows in vitro and in vivo cloning | | | | PO - 4 : | Student explains different cloning methods | | | | PO - 5 : | Student knows the theory of techniques to change, over express, knock-down and knock-out genes | | | | PO - 6 : | Student knows about the theory of techniques used in DNA and RNA quantitation. | | | | PO - 7 : | Student knows about the theory of approaches used for protein expression, purification and interaction. | | | | PO - 8 : | Student has a general idea about the computer programmes used in recombinant DNA technology | | | | PO - 9 : | Student defines area of usage of recombinant DNA technology | | | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| This course contains the topics such as; introduction to gene manipulation, biochemical reagents used for manipulation of DNA molecules (restriction enzymes, ligases, phosphotases ..etc.), and their functions, biology of vectors used in cloning (plasmid, cosmid, phasmid ..etc.), gen cloning strategies, model organisms applied for cloning, transformation and transfection of vectors into host organisms, site directed mutagenesis, quantitation of mRNA (REAL Time PCR), gene knock-out and knock-down strategies, protein expression and purification approaches, protein interactions methods, bioinformatics, applications of recombinant DNA technology. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Introduction to Recombinant DNA Technology | | | Week 2 | Biochemical reagents used for manipulation of DNA molecules and their functions.
| | | Week 3 | Biology of vectors used in cloning and their applications.
| | | Week 4 | Model organisms used in recombinant DNA technology and their comparisons.
| | | Week 5 | Gene cloning Strategies
| | | Week 6 | DNA Transfection and transformation methods for model organisms. | | | Week 7 | Site-Directed Mutagenesis, gene knock-down and knock-out strategies
| | | Week 8 | Midterm | | | Week 9 | Gene quantitation methods
| | | Week 10 | Protein expression and purification approaches | | | Week 11 | Approaches to analyse protein interactions
| | | Week 12 | Bioinformatics
| | | Week 13 | Common use areas of recombinant DNA technology | | | Week 14 | Student presentations | | | Week 15 | Student presentations | | | Week 16 | Endf of Term exam | | | |
| 1 | S.B. Primrose ve R.M. Twyman, Principles of Gene Manipulation and Genomics,Blackwell yayınevi,2006,1-4051-3544-1 | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 1 | | 2 | 30 | | Presentation | 1 | | 1 | 20 | | Homework/Assignment/Term-paper | 1 | | 1 | 10 | | End-of-term exam | 1 | | 2 | 40 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 12 | 36 | | Sınıf dışı çalışma | 3 | 3 | 9 | | Arasınav için hazırlık | 4 | 5 | 20 | | Arasınav | 1 | 2 | 2 | | Ödev | 5 | 2 | 10 | | Dönem sonu sınavı için hazırlık | 4 | 5 | 20 | | Dönem sonu sınavı | 1 | 2 | 2 | | Diğer 1 | 5 | 2 | 10 | | Total work load | | | 109 |
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