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HIS6022 | Stem Cells and Features | 2+0+2 | ECTS:7.5 | Year / Semester | Spring Semester | Level of Course | Third Cycle | Status | Elective | Department | DEPARTMENT of HISTOLOGY and EMBRYOLOGY | Prerequisites and co-requisites | None | Mode of Delivery | Face to face, Lab work | Contact Hours | 14 weeks - 2 hours of lectures and 2 hours of laboratory per week | Lecturer | -- | Co-Lecturer | None | Language of instruction | Turkish | Professional practise ( internship ) | None | | The aim of the course: | make the students learn stem cells? features and their isolation |
Programme Outcomes | CTPO | TOA | Upon successful completion of the course, the students will be able to : | | | PO - 1 : | say isolation mesenchymal stem cells from a tissue | 1,2,3,4,5,6 | 1,4,6 | PO - 2 : | explain reproduction, passage and freezing of mesenchymal stem cells | 1,2,3,4,5,6 | 1,4,6 | PO - 3 : | explain important rules on thawing and reproduction of stem cells | 1,2,3,4,5,6 | 1,4,6 | PO - 4 : | explain stem cells' differencies | 1,2,3,4,5,6 | 1,4,6 | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | |
General properties of stem cells
Stem cell plasticity
Isolation of mononuclear cells
Negative and positive selection
Tissue cell isolation
Tripsinizasyon
Basic principles in cell culture
Isolation of mesenchymal stem cells
Production of mesenchymal stem cells
CD34 + cell analysis
Freezing and storage of stem cells
Signal transduction system and transcription factors
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Course Syllabus | Week | Subject | Related Notes / Files | Week 1 | General properties of stem cells | | Week 2 | Stem cell plasticity | | Week 3 | Isolation of mononuclear cells | | Week 4 | Negative and positive selection | | Week 5 | Cell isolation from tissues | | Week 6 | Trypsinization | | Week 7 | Basic principles in cell culture | | Week 8 | Mid-term Exam | | Week 9 | Isolation of mesenchymal stem cells | | Week 10 | Production of mesenchymal stem cells | | Week 11 | CD34 + cell analysis | | Week 12 | Laboratory Exam | | Week 13 | Freezing and storage of stem cells | | Week 14 | Signal transduction system and transcription factors | | Week 16 | Final Exam | | |
1 | Ovalı E., and Karaöz E. 2004, Kök Hücreler, Trabzon | | |
1 | Ross M. H., Pawlina W., 2003, Histology A Text and Atlas, Fourth Edition, Lippincott Williams and Wilkins Company, Baltimore. | | |
Method of Assessment | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | Mid-term exam | 8 | | 1 | 30 | Laboratory exam | 12 | | 1 | 20 | End-of-term exam | 16 | | 1,5 | 50 | |
Student Work Load and its Distribution | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | Yüz yüze eğitim | 2 | 12 | 24 | Sınıf dışı çalışma | 1 | 12 | 12 | Laboratuar çalışması | 2 | 12 | 24 | Arasınav için hazırlık | 1 | 12 | 12 | Arasınav | 1 | 1 | 1 | Ödev | 4 | 12 | 48 | Proje | 1 | 1 | 1 | Dönem sonu sınavı için hazırlık | 2 | 12 | 24 | Dönem sonu sınavı | 1.5 | 1 | 1.5 | Total work load | | | 147.5 |
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