|
|
| HIS6009 | Immunohistochemical Techniques | 2+0+2 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Third Cycle | | Status | Elective | | Department | DEPARTMENT of HISTOLOGY and EMBRYOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Lab work | | Contact Hours | 14 weeks - 2 hours of lectures and 2 hours of laboratory per week | | Lecturer | -- | | Co-Lecturer | - | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | make the students learn Immunohistochemical Techniques |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | be able to explain Cytological preparations , fixation techniques | 1,3,4,5,6 | 1,4,6 | | PO - 2 : | be able to tell Immunohistochemical follow and detection methods | 1,3,4,5,6 | 1,4,6 | | PO - 3 : | be able to tell Antigen Retrival techniques | 1,3,4,5,6 | 1,4,6 | | PO - 4 : | be able to explain immunohistochemistry techniques in frozen section | 1,3,4,5,6 | 1,4,6 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Introduction to Histochemistry and cytochemistry
Cytological preparations , fixation
Immunohistochemistry definition, antigens and antibodies, primer reajantların üretimi
Immunohistochemical follow and detection methods
Histophysical method, amino acid histochemical method, enzyme histochemical method
Immunohistochemical method
Methods of detection of proteins
in situ hybridization and techniques
Antigen Retrival techniques
Immunohistochemical staining methods
Problems encountered in immunohistochemical paint
immunohistochemistry in frozen section
Matters to be considered in the selection of immunohistochemical techniques
|
| |
| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Introduction to Histochemistry and cytochemistry
| | | Week 2 | Cytological preparations , fixation
| | | Week 3 | Immunohistochemistry definition, antigens and antibodies, primer reajantların üretimi
| | | Week 4 | Immunohistochemical follow and detection methods | | | Week 5 | Histophysical method, amino acid histochemical method, enzyme histochemical method
| | | Week 6 | Immunohistochemical method
| | | Week 7 | Methods of detection of proteins | | | Week 8 | Mid-term exam | | | Week 9 | in situ hybridization and techniques | | | Week 10 | Antigen Retrival techniques | | | Week 11 | Immunohistochemical staining methods
Problems encountered in immunohistochemical paint
| | | Week 12 | lab. exam | | | Week 13 | immunohistochemistry in frozen section
| | | Week 14 | Matters to be considered in the selection of immunohistochemical techniques
| | | Week 16 | End-of-term exam | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | | 1 | 30 | | Laboratory exam | 12 | | 1 | 20 | | End-of-term exam | 16 | | 1,5 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 13 | 26 | | Laboratuar çalışması | 2 | 13 | 26 | | Arasınav için hazırlık | 1 | 13 | 13 | | Arasınav | 1 | 1 | 1 | | Kısa sınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 2 | 16 | 32 | | Dönem sonu sınavı | 1.5 | 1 | 1.5 | | Total work load | | | 100.5 |
|