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| HIS6004 | Cell and Tissue Culture | 2+0+2 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Third Cycle | | Status | Elective | | Department | DEPARTMENT of HISTOLOGY and EMBRYOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Lab work | | Contact Hours | 14 weeks - 2 hours of lectures and 2 hours of laboratory per week | | Lecturer | -- | | Co-Lecturer | - | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To make students learn Basic principles of Cell and Tissue Culture |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Be able to explain Basic principles in cell culture | 1,5,6 | 1,4,6 | | PO - 2 : | Be able to explainMethods of obtaining stem cells | 1,5,6 | 1,4,6 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Main equipments and materials that used in Cell and tissue culture laboratory
supplies and it?s contents that used in culture laboratory
Basic principles in cell culture
Get and Reproduction Culture samples
Cells snippet
Dissolution of the cells frozen and re-duplication
Light and electron microscopic evaluation of cells.
Stem cells and multipotent cell population, stem cell plasticity
Methods of obtaining stem cells
CD34 cell analysis
Production of mesenchymal cells
Mesenchymal cell markers
Usage areas of stem cell
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Main equipments and materials that used in Cell and tissue culture laboratory
| | | Week 2 | supplies and it?s contents that used in culture laboratory
| | | Week 3 | Basic principles in cell culture
| | | Week 4 | Kültür örneğinin alınıp çoğaltılması
| | | Week 5 | Cells snippet | | | Week 6 | Dissolution of the cells frozen and re-duplication
| | | Week 7 |
Light and electron microscopic evaluation of cells.
| | | Week 8 | midterm exam | | | Week 9 | Stem cells and multipotent cell population, stem cell plasticity | | | Week 10 | Methods of obtaining stem cells | | | Week 11 | CD34 cell analysis
| | | Week 12 | Lab exam | | | Week 13 | Production of mesenchymal cells
Mesenchymal cell markers
| | | Week 14 | Usage areas of stem cell
| | | Week 16 | final exam | | | |
| 1 | Karagöz ,E. Ovalı,E. 2004 Kök Hücreler,Derya Kitabevi,No 85 Trabzon | | | |
| 1 | www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Culture.html | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | | 1 | 30 | | Laboratory exam | 12 | | 1 | 20 | | End-of-term exam | 16 | | 1,5 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 12 | 24 | | Sınıf dışı çalışma | 2 | 12 | 24 | | Laboratuar çalışması | 2 | 12 | 24 | | Arasınav için hazırlık | 1 | 12 | 12 | | Arasınav | 1 | 1 | 1 | | Uygulama | 1 | 12 | 12 | | Ödev | 2 | 12 | 24 | | Kısa sınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 2 | 12 | 24 | | Dönem sonu sınavı | 1.5 | 1 | 1.5 | | Total work load | | | 147.5 |
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