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| HIS5133 | Electron Microscopy Techniques | 2+0+2 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of HISTOLOGY and EMBRYOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Lab work , Practical | | Contact Hours | 14 weeks - 2 hours of lectures and 2 hours of laboratory per week | | Lecturer | -- | | Co-Lecturer | - | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To learn the students electron microscopic techniques |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | be able to do tissue following for electron microscopy | | | | PO - 2 : | be able to do semi-thin and thin sectioning | 1,2,4 | 1,4,6 | | PO - 3 : | be able to do knives for sectioning | 1,2,4 | 1,4,6 | | PO - 4 : | be able to do contrasting process | 1,2,4 | 1,4,6 | | PO - 5 : | be able to do examination on tissue slices electron microscopically and evaluate them | 1,2,4 | 1,4,6 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) working principles
Preparation electron microscopy laboratory and matters must be noticed at working in the laboratory
Matters must be noticed during taking from animal or human tissue for electron microscopy
Fixation, fixatives selection, fixation time and chemicals are used in fixation solutions. Important rules in tissue fixation.
Tissue washing, dehydration, clearing
Plastic substance entrance into tissue and tissue blocking
Preparation of glass knives
Trimming, semi-thin sectioning (ultramikrotomi), staining semi-thin sections and examining
Grids' properties, cleaning of grids, coating the grids with film
Important points in taking thin sections
Taking the sections onto the grids
Contrasting the sections
Examining, evaluation and photographing the sections
Film bathing and card printing
Calculating magnification degrees in the photos
Fixative preparation errors in electron microscopy and the results
Tissue following errors in electron microscopy and the results
Evaluation errors in electron microscopy and the results
Follow-up clinical biopsies
Possible artifact changes in the evaluation of biopsy specimens
Possible causes of artifact formation and rules must be noticed to avoid of artifacts formation
Interpretation of biopsy results electron microscopically and reporting
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) working principles | | | Week 2 | Preparation electron microscopy laboratory and matters must be noticed at working in the laboratory | | | Week 3 | Matters must be noticed during taking from animal or human tissue for electron microscopy
Fixation, fixatives selection, fixation time and chemicals are used in fixation solutions. Important rules in tissue fixation.
| | | Week 4 | Tissue washing, dehydration, clearing | | | Week 5 | Plastic substance entrance into tissue and tissue blocking | | | Week 6 | Preparation of glass knives. Trimming, semi-thin sectioning (ultramikrotomi), staining semi-thin sections and examining | | | Week 7 | Grids's properties, cleaning of grids, coating the grids with film | | | Week 8 | Mid-term exam | | | Week 9 | Important points in taking thin sections. Taking the sections onto the grids, Contrasting the sections | | | Week 10 | Examining, evaluation and photographing the sections | | | Week 11 | Film bathing and card printing. Calculating magnification degrees in the photos | | | Week 12 | Laboratory Exam | | | Week 13 | Fixative preparation errors in electron microscopy and the results. Tissue following errors in electron microscopy and the results. Evaluation errors in electron microscopy and the results | | | Week 14 | Follow-up clinical biopsies. Possible artifact changes in the evaluation of biopsy specimens
Possible causes of artifact formation and rules must be noticed to avoid of artifacts formation. Interpretation of biopsy results electron microscopically and reporting | | | Week 16 | End-of-term exam | | | |
| 1 | Bancroft, JD, Stevens A, Turner, DR, 1996. Theory and Practice of Histological Techniques. Fourth Edition, Churchill Livingstone, New York | | | |
| 1 | Ozban N, Özmutlu Ö, 1991. Mikropreparasyon Yöntemleri, İ.Ü. Fen Fakültesi Basımevi, İstanbul | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | | 1 | 30 | | Laboratory exam | 12 | | 1 | 20 | | End-of-term exam | 16 | | 1,5 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 12 | 24 | | Sınıf dışı çalışma | 1 | 12 | 12 | | Laboratuar çalışması | 2 | 12 | 24 | | Arasınav için hazırlık | 1 | 12 | 12 | | Arasınav | 1 | 1 | 1 | | Uygulama | 2 | 12 | 24 | | Ödev | 6 | 6 | 36 | | Kısa sınav | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 2 | 12 | 24 | | Dönem sonu sınavı | 1.5 | 1 | 1.5 | | Total work load | | | 159.5 |
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