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| BIO 502 | Free Radical Biochemistry | 2+0+0 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of MEDICAL BIOCHEMISTRY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 2 hours of lectures per week | | Lecturer | Prof. Dr. Birgül KURAL | | Co-Lecturer | None | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To give the informations about the importances, types and effetcs of free radicals and the antioxidant systems against free radicals. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Counts the number of ways to the types and formations of free radicals | 1,2,3,11 | 1,3 | | PO - 2 : | Counts the types of antioxidants and explains the importance of them. | 1,2,3,11 | 1,3 | | PO - 3 : | Explains the importance of lipid peroxidation | 1,2,3,11 | 1,3 | | PO - 4 : | Explains the effects of free radicals to biomolecules | 1,2,3,11 | 1,3 | | PO - 5 : | Explain the research methods for free radicals and antioxidant system | 1,2,3,11 | 1,3 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| 1. Free radicals
1.1. Radical and non-radical oxygen species
1.2. Sources of free radicals
1.3. Transition metals and free radical production
1.4. Phagocytosis and respiratory burst
1.5. Biological effects of free radicals
1.6. Objectives of the free radical cells
1.7. Oxidant enzymes
2. Antioxidant defense systems
2.1. antioxidant enzymes
2.2. Metal-binding antioxidants
2.3. Collector, suppressor and the chain breaker antioxidants
2.4. Flavonoids
2.5. Other antioxidants
2.6. Exogenous (drugs) antioxidants
3. Lipid peroxidation
3.1. Lipid peroxidation products
3.2. Cholesterol oxidation
3.3. LDL oxidation
3.4. HDL oxidation
3.5. Lipid peroxidation and atherosclerosis
4. HDL and the oxidant-antioxidant system
5. Lipoxygenase, cyclooxygenase and isoprostanes
6. DNA oxidation
7. Protein oxidation
8. Nitric oxide and oxidant-antioxidant balance
9. Free radicals, cytokines and NF-kB
10. Diseases of free radicals
11. Free radicals and oxidation products, research methods
12. Antioxidant defense systems, research methods
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Free Radicals; Radical and non-radical oxygen derivatives | | | Week 2 | Free radical sources
Transition metals and free radical formation | | | Week 3 | Phagocytosis and respiratory burst
Biological effects of free radicals
Free radicals targets in cells | | | Week 4 | Oxidant enzymes | | | Week 5 | Antioxidant defense systems | | | Week 6 | Antioxidant defense systems | | | Week 7 | Lipid peroxidation | | | Week 8 | Mid-term exam | | | Week 9 | HDL and oxidant-antioxidant system
Lipooxygenase, cycloxygenase and isoprostanes | | | Week 10 | DNA oxidation
Protein oxidation | | | Week 11 | Nitric oxide and oxidant antioxidant balance
Free radicals, cytokines and NF-kB | | | Week 12 | Free radicals in diseases | | | Week 13 | Research methods for free radicals and oxidation products | | | Week 14 | Research methods for antioxidant systems | | | Week 16 | End-of-term exam | | | |
| 1 | Upnu RM et al., 2010; Free Radicals and Antioxidant Protocols (Methods in Molecular Biology). Humana press. 2nd Ed., Totowa | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | | 2 | 30 | | Homework/Assignment/Term-paper | 15 | | 3 | 20 | | End-of-term exam | 16 | | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 3 | 16 | 48 | | Laboratuar çalışması | 2 | 2 | 4 | | Arasınav için hazırlık | 2 | 8 | 16 | | Arasınav | 2 | 1 | 2 | | Ödev | 1 | 14 | 14 | | Dönem sonu sınavı için hazırlık | 2 | 16 | 32 | | Dönem sonu sınavı | 2 | 1 | 2 | | Total work load | | | 160 |
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