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| BIO 531 | Research Techniques in Biochemistry - I | 3+0+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Compulsory | | Department | DEPARTMENT of MEDICAL BIOCHEMISTRY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | -- | | Co-Lecturer | Assoc. Prof. Dr. Ahmet ALVER | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | The course aims to provide the students with a general knowledge on the theory of techniques used in biochemistry. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | learn biochemical methods | 4 | 1,3, | | PO - 2 : | learn biochemical laboratory methods in human health | 4 | 1,3 | | PO - 3 : | apply biochemical methods | 4 | 1,3 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Physical and chemical feature of waterpH and buffers: Definition, preparation and featuresBuffer systems in human bodySpectrophotometrySpectrophotometric measurements and enzyme assaysImmunoassays |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Introduction | | | Week 2 | Water supply on laboratories | | | Week 3 | Laboratory supplies | | | Week 4 | Centrifugation | | | Week 5 | Buffers | | | Week 6 | Spectrophotometry | | | Week 7 | Spectrophotometry | | | Week 8 | Mid-term exam | | | Week 9 | Spectrophotometry | | | Week 10 | Spectrophotometry | | | Week 11 | Spectrophotometry | | | Week 12 | Spectrophotometry /Homework | | | Week 13 | Immunoassays | | | Week 14 | Immunoassays | | | Week 16 | End-of-term exam | | | |
| 1 | Robyt,JF., White BJ.1987;Biochemical Techniques,Waveland Press, Illinois | | | 2 | Boyer, RF.1993;Modern Experimental Biochemistry, The Benjamin /Cummings Publ.,California | | | 3 | Barker,K.1998;At The Bench, Cold Spring harbor Lab Press, New York | | | |
| 1 | Pingoud,A.,Urbanke,C,Hoggett,J.Jeltsch,A.2002; Biochemical Methods, Wiley-VCH,Weinheim | | | 2 | Metzler, D. 2001; Biochemistry, Vol.1, Harcourt-Academic Press,San Diego | | | 3 | Metzler, D.2003; Biochemistry, Vol.2, Harcourt-Academic Press,San Diego | | | 4 | Roskams,J., Rodgers,L.2002;Lab Ref,Cold Spring harbor Lab Press, New York | | | 5 | Mellick A.S., Rodgers,L.2007;Lab Ref,vol.2,Cold Spring harbor Lab Press, New York | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | | 2 | 30 | | Homework/Assignment/Term-paper | 12 | | 4 | 20 | | End-of-term exam | 18 | | 3 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 3 | 14 | 42 | | Laboratuar çalışması | 0 | 0 | 0 | | Arasınav için hazırlık | 20 | 2 | 40 | | Arasınav | 2 | 1 | 2 | | Uygulama | 0 | 0 | 0 | | Klinik Uygulama | 0 | 0 | 0 | | Ödev | 5 | 3 | 15 | | Proje | 0 | 0 | 0 | | Kısa sınav | 0 | 0 | 0 | | Dönem sonu sınavı için hazırlık | 30 | 2 | 60 | | Dönem sonu sınavı | 24 | 1 | 24 | | Diğer 1 | 0 | 0 | 0 | | Diğer 2 | 0 | 0 | 0 | | Total work load | | | 225 |
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