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| TMIK618 | Special Topics in Genetics | 2+0+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Third Cycle | | Status | Elective | | Department | DEPARTMENT of MEDICAL MICROBIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Lab work , Practical | | Contact Hours | 14 weeks - 2 hours of lectures per week | | Lecturer | Prof. Dr. Ali Osman KILIÇ | | Co-Lecturer | Asst. Prof. Dr. C. Kurtuluş BURUK | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Revision of genetics and molecular applications with special in vitro nucleic amplification technics. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | argue of genetical analysis methods | 1,2,3,4,5,6,7,8 | | | PO - 2 : | summarize the structure and function of nucleic acids. | 1,2,3,4,5,6,7,8 | 1,6 | | PO - 3 : | manage isolation methods of nucleic acids | 1,2,3,4,5,6,7,8 | 1,6 | | PO - 4 : | know principles and application areas of Polymerase Chain Reaction | 1,2,3,4,5,6,7,8 | 1,6 | | PO - 5 : | design a Polymerase Chain Reaction and complete the reaction | 1 | 1,6 | | PO - 6 : | give information on Southern Blotting | 1,2,3,4,5,6,7,8 | 1,6 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Structure and Replication of DNA molecules, Isolation of nucleic acids, In vitro amplification methods of nucleic acids: Polymerase Chain Reaction (PCR), Application and use of PCR, Sort af PCR.
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Structure of DNA | | | Week 2 | Replication of DNA | | | Week 3 | Methods for isolation of nucleic acids | | | Week 4 | DNA amplification methods | | | Week 5 | What is Polymerase Chain Reaction? | | | Week 6 | PCR primers | | | Week 7 | Preparation of reaction mixes | | | Week 8 | Mid-term exam | | | Week 9 | Amplification and analysis of PCR products | | | Week 10 | Real time PCR | | | Week 11 | Optimisation and adaptation of PCR | | | Week 12 | Assessment of homework | | | Week 13 | Ensuring PCR quality | | | Week 14 | Variants os the standart PCR protocols and PCR in diagnosis of infectious agents | | | Week 16 | End-of-term exam | | | |
| 1 | van Pelt-Verkuil E., an Belkum A. , Hays J.P. 2008; Principles and Technical Aspects of PCR Amplification, Springer. | | | |
| 1 | Persing, D. H., Tenover, F. C., Versalovic, J., Tang YiWei, Unger, E. R., Relman, D. A., White, T. J. 2006; Molecular microbiology: diagnostic principles and practice, ASM Press, Washington, D.C. Çevirisi: Tekeli A. ve Ustaçelebi Ş. (çeviri editörleri) 2006; Moleküler Mikrobiyoloji: Tanı Prensipleri ve Uygulamalar, Palme Yayıncılık, Ankara. | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | | 1 | 30 | | Homework/Assignment/Term-paper | 12 | | 1 | 20 | | End-of-term exam | 17 | | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 13 | 26 | | Sınıf dışı çalışma | 2 | 14 | 28 | | Laboratuar çalışması | 3 | 15 | 45 | | Arasınav için hazırlık | 2 | 7 | 14 | | Arasınav | 1 | 1 | 1 | | Uygulama | 1 | 10 | 10 | | Ödev | 2 | 10 | 20 | | Dönem sonu sınavı için hazırlık | 8 | 1 | 8 | | Dönem sonu sınavı | 1 | 1 | 1 | | Total work load | | | 153 |
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