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GRADUATE INSTITUTE of HEALTH SCIENCES / DEPARTMENT of MEDICAL MICROBIOLOGY
Masters with Thesis
Course Catalog
https://www.ktu.edu.tr/mikrobiyoloji
Phone: +90 0462 0 462 377 5110
SABE
GRADUATE INSTITUTE of HEALTH SCIENCES / DEPARTMENT of MEDICAL MICROBIOLOGY / Masters with Thesis
Katalog Ana Sayfa
  Katalog Ana Sayfa  KTÜ Ana Sayfa   Katalog Ana Sayfa
 
 
BIO 532Research Techniques in Biochemistry - II3+0+0ECTS:7.5
Year / SemesterSpring Semester
Level of CourseSecond Cycle
Status Elective
DepartmentDEPARTMENT of MEDICAL MICROBIOLOGY
Prerequisites and co-requisitesNone
Mode of DeliveryFace to face
Contact Hours14 weeks - 3 hours of lectures per week
Lecturer--
Co-LecturerAssoc. Prof. Dr. Ahmet ALVER
Language of instructionTurkish
Professional practise ( internship ) None
 
The aim of the course:
The course aims to provide the students with a general knowledge on the theory of techniques used in biochemistry
 
Programme OutcomesCTPOTOA
Upon successful completion of the course, the students will be able to :
PO - 1 : know biochemical methods1,2,3,41,3
PO - 2 : learn biochemical laboratory methods in human health1,2,3,41,3
PO - 3 : apply biochemical methods1,2,3,41,3
CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome

 
Contents of the Course
Principles of Chromatography Elution Methods TLC/Paper Chromatography Ion-Exchange Chromatography Gel Filtration Affinity Chromatography Hidrofobic Interaction Chromatography Principles of Electrophoresis Support Media Polyacrilamide gel (SDS-PAGE) Blotting Protein Electrophoresis in Clinical Diagnosis
 
Course Syllabus
 WeekSubjectRelated Notes / Files
 Week 1Definitions/Concepts of Proteomics
 Week 2Elution Methods
 Week 3TLC/Paper chromatography/HPLC
 Week 4Ion-Exchange Chromatography
 Week 5Gel filtration
 Week 6Affinity Chromatography
 Week 7Hidrofobic Interaction Chromatography
 Week 8Mid-term exam
 Week 9Principles of Electrophoresis
 Week 10Support media
 Week 11Polyacrilamide gel (SDS-PAGE)
 Week 12Continuous/Discontinuous Systems /Homework
 Week 13Blotting
 Week 14Protein Electrophoresis in Clinical Diagnosis
 Week 16End-of-term exam
 
Textbook / Material
1Robyt,JF., White BJ.1987;Biochemical Techniques,Waveland Press, Illinois
2Boyer, RF.1993;Modern Experimental Biochemistry, The Benjamin /Cummings Publ.,California
3Barker,K.1998;At The Bench, Cold Spring harbor Lab Press, New York
 
Recommended Reading
1Pingoud,A.,Urbanke,C,Hoggett,J.Jeltsch,A.2002; Biochemical Methods, Wiley-VCH,Weinheim
2Metzler, D. 2001; Biochemistry, Vol.1, Harcourt-Academic Press,San Diego
3Metzler, D. 2003; Biochemistry, Vol.2, Harcourt-Academic Press,San Diego
4Barker,K.1998;At The Bench, Cold Spring harbor Lab Press, New York
5Roskams,J., Rodgers,L.2002;Lab Ref,Cold Spring harbor Lab Press, New York
 
Method of Assessment
Type of assessmentWeek NoDate

Duration (hours)Weight (%)
Mid-term exam 8 2 30
Homework/Assignment/Term-paper 12 4 20
End-of-term exam 17 3 50
 
Student Work Load and its Distribution
Type of workDuration (hours pw)

No of weeks / Number of activity

Hours in total per term
Yüz yüze eğitim 3 14 42
Sınıf dışı çalışma 6 14 84
Laboratuar çalışması 0 0 0
Arasınav için hazırlık 3 14 42
Arasınav 2 1 2
Uygulama 0 0 0
Klinik Uygulama 0 0 0
Ödev 6 2 12
Proje 0 0 0
Kısa sınav 0 0 0
Dönem sonu sınavı için hazırlık 2 14 28
Dönem sonu sınavı 3 1 3
Diğer 1 0 0 0
Diğer 2 0 0 0
Total work load213