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| BIYI7143 | Molecular Biomethods | 3+0+0 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Third Cycle | | Status | Elective | | Department | DEPARTMENT of BIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Prof. Dr. Ali Osman BELDÜZ | | Co-Lecturer | None | | Language of instruction | | | Professional practise ( internship ) | None | | | | The aim of the course: | | The main objectives of this course are to provide the student with a clear presentation of the basic concepts, recent and important molecular biology techniques and principles of related subjects. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | understand and apply practical techniques used in fundamental and advanced biotechnology
| 1,3,5,6 | 1,3,5, | | PO - 2 : | understand the basic theory and background behind biotechnology
techniques | 1,3,5,6 | 1,3,5, | | PO - 3 : | demonstrate the ability to find and understand pertinent scientific information | 1,3,5,6 | 1,3,5, | | PO - 4 : | explain principles of analyses methods of nucleic acids and proteins | 1,3,5,6 | 1,3,5, | | PO - 5 : | appreciate the applications and implications of recombinant DNA technology in medicine and industry | 1,3,5,6 | 1,3,5 | | PO - 6 : | demonstrate an ability to critically analyze experimental data | 1,3,5,6 | 1,3,5, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Equipment And Chemicals For Molecular Research Laboratory, DNA and RNA Isolation Techniques, Gel Electrophoresis, Blotting Techniques, PCR Techniques And Futures, DNA Sequencing, RFLP, Hybridization Techniques, Mutation Analysing Techniques, ELISA, Cell Culturing And Stem Cell Techniques, Cancer, Cytogenetic Techniques, FISH, HLA Tissue Separation, Flow Cytometric Analysis. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Probe Design, Production, and Applications | | | Week 2 | Capillary Electrophoresis of DNA:Biomedical Applications
| | | Week 3 | Protein Electrophoresis
| | | Week 4 | Mass Spectrometry of Proteins and Peptides
| | | Week 5 | Post-Translational Modifications of Proteins
| | | Week 6 | Protein Engineering Directed Protein Evolution | | | Week 7 | Ion-Exchange Chromatography | | | Week 8 | Size-Exclusion Chromatography | | | Week 9 | midterm exam
| | | Week 10 | Size-Exclusion Chromatography
| | | Week 11 | Hydrophobic Interaction Chromatography
| | | Week 12 | Affinity Chromatography
| | | Week 13 | High Performance Liquid Chromatography (HPLC) of Peptides and Proteins..
| | | Week 14 | Cryopreservation | | | Week 15 | Electron Microscopy | | | Week 16 | End-of-term exam | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 21/04/2022 | 1,5 | 30 | | In-term studies (second mid-term exam) | 13 | 12/05/2022 | 1 | 10 | | Oral exam | 15 | 30/05/2022 | 0,5 | 5 | | Presentation | 15 | 30/05/2022 | 1 | 5 | | End-of-term exam | 16 | 9/06/2022 | 1,5 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 5 | 13 | 65 | | Laboratuar çalışması | 0 | 0 | 0 | | Arasınav için hazırlık | 6 | 7 | 42 | | Arasınav | 2 | 1 | 2 | | Uygulama | 0 | 0 | 0 | | Klinik Uygulama | 0 | 0 | 0 | | Ödev | 15 | 1 | 15 | | Proje | 0 | 0 | 0 | | Kısa sınav | 2 | 7 | 14 | | Dönem sonu sınavı için hazırlık | 6 | 7 | 42 | | Dönem sonu sınavı | 2 | 1 | 2 | | Diğer 1 | 1 | 1 | 1 | | Diğer 2 | 0 | 0 | 0 | | Total work load | | | 225 |
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