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| BIY7141 | Nucleic Acids | 3+0+0 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Third Cycle | | Status | Elective | | Department | DEPARTMENT of BIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Prof. Dr. Ali Osman BELDÜZ | | Co-Lecturer | None | | Language of instruction | | | Professional practise ( internship ) | None | | | | The aim of the course: | | This course aims to make the student ready for a molecular biology researches by equipping them well on gene cloning methods, modification enzymes, basic molecular biological techniques, cloning and expression in proper prokaryotic and eukaryotic vectors, gene expression in prokaryotes and yeast and things needs to be paid attention for a good expression. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | understand the structure and functioning of selected plasmids and phages, particularly in regard replication mechanisms | 1,5,6,8 | 1,2,5, | | PO - 2 : | understand the important features of vectors used in gene cloning, the enzymology of gene cloning | 1,5,6,8 | 1,2,5, | | PO - 3 : | understand control of gene expression in selected prokaryotic and eukaryotic systems | 1,5,6,8 | 1,2,5, | | PO - 4 : | understand the recent mutagenesis methodes of the genes, the strategies used in the construction and screening of gene libraries and in the analysis of cloned genes | 1,5,6,8 | 1,2,5, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Isolation and idendification of DNA fragments. Ligation, plasmid vectors, cloning in procaryotes and eucaryotes. Gene libraries. Idendification of recombinant dna. Mutagenesis. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Basic Nucleic acid techniques: Gel Electrophoresis, DNA Sequencing, Pyrosequencing | | | Week 2 | Basic Nucleic acid techniques:Oligonucmeotide synthesis, PCR and PCR types | | | Week 3 | Basic Nucleic acid techniques:Restriction and Modification enzymes, DNA restriction and ligation into a vector, in vitro recombination | | | Week 4 | Basic Biology of plasmid and phase vectors | | | Week 5 | Basic Biology of plasmid and phase vectors | | | Week 6 | Cosmids, phagemids and other advanced vectors | | | Week 7 | Cosmids, phagemids and other advanced vectors | | | Week 8 | Construction of DNA libraries and screening | | | Week 9 | Mid-term exam | | | Week 10 | Sequences to simplify the protein purifications, HisTaq, intein, extein | | | Week 11 | Inclution body formations and solutions for solubilizations | | | Week 12 | Strategies for gene cloning and overexpression | | | Week 13 | Strategies for gene cloning and overexpression; homework and project presentations | | | Week 14 | Gen Mutations: Single Nucleotide mutations and Protein Engineering;homework and project presentations | | | Week 15 | Gen Mutations: Single Nucleotide mutations and Protein Engineering;homework and project presentations | | | Week 16 | End-of-term exam | | | |
| 1 | Principles of Gene Manipulation, Sandy B. Primrose, Giuseppe Bertola, Bob Old, Richard Twyman, Rev 7th. Ed. December 2005, Paperback, 512pp, ISBN: 1405135441, Blackwell Publishers.
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| 1 | Gene cloning, Jula Lodge, Pete Lund & Steve MinchinNew York, NY : Taylor& Francis, 2007. ISBN: 0-7487-6534-4
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| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | | 1,5 | 30 | | Oral exam | 15 | | 0,5 | 10 | | Presentation | 15 | | 1 | 10 | | End-of-term exam | 16 | | 1,5 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 5 | 14 | 70 | | Laboratuar çalışması | 0 | 0 | 0 | | Arasınav için hazırlık | 2 | 7 | 14 | | Arasınav | 2 | 1 | 2 | | Uygulama | 0 | 0 | 0 | | Klinik Uygulama | 0 | 0 | 0 | | Ödev | 8 | 1 | 8 | | Proje | 0 | 1 | 0 | | Kısa sınav | 3 | 7 | 21 | | Dönem sonu sınavı için hazırlık | 9 | 7 | 63 | | Dönem sonu sınavı | 2 | 1 | 2 | | Diğer 1 | 1 | 1 | 1 | | Diğer 2 | 1 | 1 | 1 | | Total work load | | | 224 |
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