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| BIYL7142 | Extrachromosomal Elements | 3+0+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Third Cycle | | Status | Elective | | Department | DEPARTMENT of BIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Dr. Öğr. Üyesi Fulya AY | | Co-Lecturer | Prof. Dr. Ali Osman BELDUZ | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Increasing the students' knowledge on extrachromosomal DNA elements and prepare them for a graduate study. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | explain the extrachromosomal elements | 1,3 | 1,5 | | PO - 2 : | discover the usage of plasmids as cloning vectors | 1,3 | 1,5 | | PO - 3 : | discover the usage of plasmids as expression vectors | 1,3 | 1,5 | | PO - 4 : | discover the usage of plasmids at disease's therapy | 1,3 | 1,5 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| An overview on extrachromosomal elements, plasmids, transposons, mitochondri and chloroplast DNA's, E. coli plasmids, the scientific background of the plasmids used routinely to clone genes and to express proteins, archaeal and eukaryotic plasmids, plasmid-based expression systems for mammalian cells, bioinformatics tools and methods for plasmid sequence analysis, horizontal gene transfer mediated by plasmids, plasmids for gene therapy, plasmid DNA for cancer and infectious diseases. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Procaryotic and eucaryotic cell structure | | | Week 2 | An overview on extrachromosomal elements | | | Week 3 | Plasmids; cloning plasmids | | | Week 4 | Transposons | | | Week 5 | Mitochondri DNA | | | Week 6 | Chloroplast DNA | | | Week 7 | Constructing plasmids | | | Week 8 | Archael and eucaryotic plasmids | | | Week 9 | Mid-term exam | | | Week 10 | Expression vectors | | | Week 11 | Student presentations | | | Week 12 | Student presentations | | | Week 13 | Medical usage of plasmids | | | Week 14 | Sequence analysis of plasmids | | | Week 15 | Horizantal gene transfer by plasmids | | | Week 16 | End-of-term exam | | | |
| 1 | Wickner, R.; Extrachromosomal Elements in Lower Eukaryotes; Springer; 1997 | | | |
| 1 | Phillips, G., Phillips, G. J.; Plasmid Biology; Amer Society for Microbiology, 2004 | | | 2 | Summers, K.D.; The Biology of Plasmids;Blackwell Pub; 1996 | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 20/11/2013 | 1 | 30 | | Presentation | 12 | 11/12/2013 | 1 | 20 | | End-of-term exam | 16 | 08/01/2014 | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 5 | 14 | 70 | | Laboratuar çalışması | 0 | 0 | 0 | | Arasınav için hazırlık | 7 | 7 | 49 | | Arasınav | 2 | 1 | 2 | | Uygulama | 0 | 0 | 0 | | Klinik Uygulama | 0 | 0 | 0 | | Ödev | 2 | 5 | 10 | | Proje | 2 | 7 | 14 | | Kısa sınav | 0 | 5 | 0 | | Dönem sonu sınavı için hazırlık | 6 | 6 | 36 | | Dönem sonu sınavı | 1 | 1 | 1 | | Diğer 1 | 0 | 11 | 0 | | Diğer 2 | 0 | 0 | 0 | | Total work load | | | 224 |
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