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| BIY7330 | Practical Errors in Molecular Technics | 3+0+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Third Cycle | | Status | Elective | | Department | DEPARTMENT of BIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Prof. Dr. Sabriye ÇANAKÇI | | Co-Lecturer | Prof. Dr. Ali Osman BELDUZ | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To inform students some errors and solutions of the some technics of molecular biology |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | know the preparation of buffers and other solutions | 2,5,6 | 1,5, | | PO - 2 : | explain purification of DNA and RNA | 2,5,6 | 1,5, | | PO - 3 : | explain PCR, electrophoresis, nucleic acid hybridization and expression systems | 2,5,6 | 1,5, | | PO - 4 : | explain theoretical faults and solutions made in some technics in molecular biology
| 2,5,6 | 1,5, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Getting what you need from a supplier , the preparation of buffers and other solutions, working safely with biological samples and radioactive materials, DNA and RNA purification, PCR, electrophoresis, nucleic acid hybridization, expression systems, theoretical faults and solutions made in these technics. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | The Preparation of Buffers and Other Solutions | | | Week 2 | Working Safely with Biological Samples | | | Week 3 | DNA purification | | | Week 4 | RNA purification | | | Week 5 | Electrophoresis | | | Week 6 | Nucleotides, Oligonucleotides, and Polynucleotides | | | Week 7 | PCR | | | Week 8 | Restriction Endonucleases | | | Week 9 | Mid-term exam | | | Week 10 | E. coli Expression Systems | | | Week 11 | Student seminar | | | Week 12 | Student seminar | | | Week 13 | Student seminar | | | Week 14 | Eukaryotic Expression | | | Week 15 | Nucleic Acid Hybridization | | | Week 16 | End-of-term exam | | | |
| 1 | Alan S. Gerstein, 2001, Molecular Biology Problem Solver: A Laboratory Guide, Wiley-Liss, Inc. | | | |
| 1 | Yıldırım, A., Bardakçı, F., Karataş, M., Tanyolaç, B., 2007, Moleküler Biyoloji, Nobel, Ankara | | | 2 | Madigan, M.T., Martinko, J.M., Çeviri Editötü: Çökmüş, C. 2010, Brook Mikroorganizmaların Biyolojisi, Palme Yayıncılık, Ankara | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 19/11/2018 | 1 | 30 | | Presentation | 12 | 10/12/2018 | 1 | 20 | | End-of-term exam | 16 | 14/01/2019 | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 5 | 14 | 70 | | Arasınav için hazırlık | 30 | 1 | 30 | | Arasınav | 1.5 | 1 | 1.5 | | Ödev | 42.5 | 1 | 42.5 | | Dönem sonu sınavı için hazırlık | 37.5 | 1 | 37.5 | | Dönem sonu sınavı | 1.5 | 1 | 1.5 | | Total work load | | | 225 |
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