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GRADUATE INSTITUTE of NATURAL and APPLIED SCIENCES / DEPARTMENT of BIOLOGY
Masters with Thesis
Course Catalog
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Phone: +90 0462 3772522 , 3253200
FBE
GRADUATE INSTITUTE of NATURAL and APPLIED SCIENCES / DEPARTMENT of BIOLOGY / Masters with Thesis
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BIY5400Preparat Techniques in Biology2+0+2ECTS:7.5
Year / SemesterFall Semester
Level of CourseSecond Cycle
Status Elective
DepartmentDEPARTMENT of BIOLOGY
Prerequisites and co-requisitesNone
Mode of DeliveryFace to face, Lab work
Contact Hours14 weeks - 2 hours of lectures and 2 hours of laboratory per week
LecturerProf. Dr. Hüseyin İNCEER
Co-LecturerNone
Language of instructionTurkish
Professional practise ( internship ) None
 
The aim of the course:
The course aims to introduce a basic methods of doing the slides of biological materials
 
Programme OutcomesCTPOTOA
Upon successful completion of the course, the students will be able to :
PO - 1 : prepare biological materials for preparations1,2,3,4,5,61
PO - 2 : understand preparation methods1,2,3,4,5,61
PO - 3 : prepare the slides from biological materials1,2,3,4,5,64
PO - 4 : take digital fotographs from the slides1,2,3,4,5,64
CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome

 
Contents of the Course
Material collection. Fixation and preservation of the material. Dehydration. Preparation of paraffin blocks. Sectioning by microtome. Stains used for staining of cells and tissues. Selection of desired stains. Staining of sections and preparation of indefinite sections.
 
Course Syllabus
 WeekSubjectRelated Notes / Files
 Week 1Principlers of laboratory working
 Week 2Microscopies
 Week 3Material collection. Fixation and preservation of the material
 Week 4Biological dyes
 Week 5Preparation of paraffin blocks
 Week 6Sectioning by microtome
 Week 7Staining of sections
 Week 8Preparation of permanent slides
 Week 9Mid-term exam
 Week 10Laboratory working
 Week 11Laboratory exam
 Week 12Seminar
 Week 13Laboratory working
 Week 14Laboratory working
 Week 15Laboratory working
 Week 16End-of-term exam
 
Textbook / Material
1Algan, G. 1981; Bitkisel dokular için Mikroteknik, Fırat Üniversitesi Yayınları, No 1, Elazığ
 
Recommended Reading
1Elçi, Ş. 1994; Sitogenetikte araştırma yöntemleri ve metodlar, 100. Yıl Üniversitesi Yayınları, No 18, Van
 
Method of Assessment
Type of assessmentWeek NoDate

Duration (hours)Weight (%)
Mid-term exam 9 19/11/2013 1 30
Laboratory exam 11 04/12/2013 1,5 10
Presentation 12 11/12/2013 1 5
Homework/Assignment/Term-paper 4
7
16/10/2013
06/11/2013
3
3
5
End-of-term exam 16 08/01/2014 1 50
 
Student Work Load and its Distribution
Type of workDuration (hours pw)

No of weeks / Number of activity

Hours in total per term
Yüz yüze eğitim 2 14 28
Sınıf dışı çalışma 1 13 13
Laboratuar çalışması 2 14 28
Arasınav için hazırlık 18 1 18
Arasınav 1 1 1
Ödev 5 6 30
Dönem sonu sınavı için hazırlık 25 2 50
Diğer 1 16 2 32
Total work load200