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| KIM7270 | Structural Characterization of Industrial Enz | 3+0+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Third Cycle | | Status | Elective | | Department | DEPARTMENT of CHEMISTRY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | -- | | Co-Lecturer | Prof.Dr. Ahmet ÇOLAK | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To understand of Enzyme kinetic, To teach the effect of different parameters on enzyme activity and stability and discussion of structure |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | understand enzyme kinetic and explain the effect of some parameters on enzyme activity | 1,2,3 | 1 | | PO - 2 : | explain the protein structure by using the data obtained from characterization studies | 1,2,3 | 1,3 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Determination of industrial enzyme activity, investigation of the effect of protein concentration on enzyme activity, the effect of substrate concentration, pH, temperature, inhibitors and matel ions on enzyme activity, investigation of the thermal stability of an enzyme, electrophoretic determination of proteins, discussion of protein structure with data |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Determination of the activity of an industrial enzyme | | | Week 2 | Investigation of the effect of protein concentration on enzyme activity | | | Week 3 | The effect of substrate concentration on the enzyme activity | | | Week 4 | Investigation of enzyme inhibition | | | Week 5 | The effect of metal ions on the enzyme activity | | | Week 6 | The effect of pH on the enzyme activity | | | Week 7 | The effect of temperature on the enzyme activity | | | Week 8 | Investigation of the thermal stability of an enzyme | | | Week 9 | Mid-term exam | | | Week 10 | Investigation of the thermal stability of an enzyme | | | Week 11 | Electrophoretic determination of proteins | | | Week 12 | Electrophoretic determination of proteins | | | Week 13 | II. Examination | | | Week 14 | Discussion of protein structure with data | | | Week 15 | Discussion of protein structure with data. Presentation. | | | Week 16 | End-of-term exam | | | |
| 1 | Fersht, A. 1985; Enzyme Structure and Mechanism, W.H. Freeman and Company, New York. | | | 2 | Palmer, T. 1994; Enzim Bilgisi, Bilimsel ve Teknik Yayınları Çeviri Vakfı, İstanbul. | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 22/11/2015 | 1,5 | 25 | | Presentation | 15 | 08.01.2016 | 1 | 25 | | End-of-term exam | 16 | 17/01/2016 | 1,5 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 2 | 14 | 28 | | Arasınav için hazırlık | 10 | 1 | 10 | | Arasınav | 1.5 | 1 | 1.5 | | Ödev | 0 | 14 | 0 | | Dönem sonu sınavı için hazırlık | 10 | 1 | 10 | | Dönem sonu sınavı | 1 | 1 | 1 | | Total work load | | | 92.5 |
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