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| KIML5710 | Biological Activity Determinations | 3+0+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of CHEMISTRY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | -- | | Co-Lecturer | Lecturers in Biochemistry Division | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To understand the methods used in biological activity (bioactivity) investigations. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | know the bioactivity terminology | 1,3 | 1,3,5,6 | | PO - 2 : | know the method development strategies in bioactivity determinations | 1,3 | 1,3,5,6 | | PO - 3 : | know how to prepare a project involving bioactivity area | 1,3 | 1,3,5,6 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| The scope of the biological activity investigations, mechanisms of antioxidant activity, methodology of antioxidant activity determinations, common antioxidant activity determination methods, general overview of antimicrobial activities, antibacterial activity determination methods, antifungal activity methods, antiviral activity methods, approaches for the effect against cancer, antitumor activity determination methods, tumoricidal activity determination methods, tumor preventive activity determinations, evaluation of enzyme inhibition with respect to bioactive compounds, enzyme inhibitors used as drug, bioactivity determination methods based on enzyme inhibition, agonist and antagonist activity determination methods, biomolecule stability increasing activity determinations. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Scope of biological activity investigations | | | Week 2 | Antioxidant activity mechanisms | | | Week 3 | Antioxidant activity determination methodology | | | Week 4 | Commonly used antioxidant activity determination methods | | | Week 5 | General view at antimicrobial activities | | | Week 6 | Antibacterial and antifungal activity determination methods | | | Week 7 | Antiviral activity methods | | | Week 8 | Mid-term exam | | | Week 9 | Approaches for the effects against cancer, antitumor activity determination methods, tumoricidal activity determination methods, tumor preventive activity determinations | | | Week 10 | Evaluation of enzyme inhibition based on bioactive compounds | | | Week 11 | Enzyme inhibitors used as drug | | | Week 12 | Bioactivity determination methods based on enzyme inhibition | | | Week 13 | Agonist and antagonist activity determination methods | | | Week 14 | Biomolecule stabilizing activity methods | | | Week 15 | Timely examples about literature on the methods used in bioactivity investigations | | | Week 16 | End-of-term exam | | | |
| 1 | Shiqi Peng, Ming Zhao, 2009. Pharmaceutical Bioassays: Methods and Applications, Wiley. | | | |
| 1 | Donald Armstrong (Editor), 2002. Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (Methods in Molecular Biology), Humana Pres. | | | 2 | Donald Armstrong (Editor), 2002. Oxidative Stress and Antioxidant Protocols (Methods in Molecular Biology), Humana Pres. | | | 3 | Rao M. Uppu (Editor), Subramanyam N. Murthy (Editor), William A. Pryor (Editor), Narasimham L. Parinandi (Editor), 2009. Free Radicals and Antioxidant Protocols (Methods in Molecular Biology), Humana Pres. | | | 4 | Maria Lis-Balchin, 1999. Aroma Science: The Chemistry and Bioactivity of Essential Oils, Amberwood Publishing. | | | 5 | D.S. Ed. Reeves, 1999. Clinical Antimicrobial Assays, Oxford University Pres, İngiltere. | | | 6 | Brian M. Lawrence, 2005. Antimicrobial Biological Activity of Essential Oils, Allured Pub Corp. | | | 7 | D.S. Reeves, I. Phillips, J.D. Williams, R. Wise, 1978. Laboratory Methods in Antimicrobial Chemotherapy, Churchill Livingstone. | | | 8 | John K. Buolamwini (Editor), Alex A. Adjei (Editor), 2003. Novel Anticancer Drug Protocols (Methods in Molecular Medicine), Humana Pres. | | | 9 | John McBrewster (Editor), Frederic P. Miller (Editor), Agnes F. Vandome (Editor), 2009. Enzyme inhibitor: Enzyme, Enzyme assay, Competitive inhibition, Uncompetitive inhibitor, Mixed inhibition, Non-competitive inhibition, Enzyme activator, Alphascript Publishing. | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | 29/03/2016 | 90 | 30 | | Project | 13 | 05/05/2016 | 120 | 10 | | Practice | 14 | 12/05/2016 | 60 | 10 | | End-of-term exam | 16 | 27/05/201 | 90 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 7 | 14 | 98 | | Arasınav için hazırlık | 10 | 1 | 10 | | Arasınav | 2 | 1 | 2 | | Ödev | 2 | 7 | 14 | | Proje | 20 | 1 | 20 | | Dönem sonu sınavı için hazırlık | 12 | 1 | 12 | | Dönem sonu sınavı | 2 | 1 | 2 | | Total work load | | | 200 |
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