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| KIM5530 | Techniques in Protein Chemistry | 3+0+0 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of CHEMISTRY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | -- | | Co-Lecturer | Lecturers in Biochemistry Division | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | To understand structure and organization of proteins, and techniques used in isolation and purification of proteins. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | know structural and functional properties of proteins | 1 | 1,3,5,6 | | PO - 2 : | know protein purification methods | 1,3 | 1,3,5,6 | | PO - 3 : | know how a protein/proteins will be purified from a material by following successively suitable steps | 1,3 | 1,3,5,6 | | PO - 4 : | determine the process they will follow during purification study | 1 | 1,3,5,6 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Protein structure. Techniques in protein isolation and purification. Protein purification steps. Centrifugation and methods depending on solubility. Chromatographic and electrophoretic methods. Characterization of proteins. Methods for determination of protein concentration. Determination of protein structure. Function of proteins and enzymes. Activity Measurement Methods. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Protein structure | | | Week 2 | Techniques in protein isolation and purification | | | Week 3 | Steps in protein purification | | | Week 4 | Centrifugation, dialysis, and techniques based on solubility | | | Week 5 | Chromatographic methods | | | Week 6 | Electrophoretic methods | | | Week 7 | Protein characterization | | | Week 8 | Determination of protein size and shape | | | Week 9 | Mid-term exam | | | Week 10 | Proteinlerin spektroskopik özellikleri | | | Week 11 | Conformations analysis and primary structure determination of proteins | | | Week 12 | Chemical synthesis of peptides | | | Week 13 | Protein functions and enzymes | | | Week 14 | Activity measurement methods | | | Week 15 | Timely examples about literature on the methods used in protein chemistry | | | Week 16 | End-of-term exam | | | |
| 1 | A. Telefoncu, 1996; Proteinlerin Saflaştırılması ve Karakterizasyonu, Biyokimya Lisansüstü Yaz Okulu. | | | 2 | G. Temizkan ve N. Arda, 2004; Moleküler Biyolojide Kullanılan Yöntemler (2nd Edition), Nobel Tıp Kitabevleri. | | | 3 | G. Walsh, 2002; Proteins: Biochemistry and Biotechnology, Chichester:John Wiley and Sons. | | | |
| 1 | M. Rosenberg, 1996; Protein analysis and purification : benchtop techniques, Boston: Birkhauser. | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 06/04/2019 | 1,5 | 25 | | Presentation | 15 | 18/05/2019 | 1 | 25 | | End-of-term exam | 16 | 25/05/2019 | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 3 | 14 | 42 | | Laboratuar çalışması | 0 | 0 | 0 | | Arasınav için hazırlık | 4 | 8 | 32 | | Arasınav | 3 | 2 | 6 | | Uygulama | 0 | 0 | 0 | | Klinik Uygulama | 0 | 0 | 0 | | Ödev | 5 | 14 | 70 | | Proje | 0 | 0 | 0 | | Kısa sınav | 0 | 0 | 0 | | Dönem sonu sınavı için hazırlık | 3 | 14 | 42 | | Dönem sonu sınavı | 1 | 1 | 1 | | Diğer 1 | 0 | 0 | 0 | | Diğer 2 | 0 | 0 | 0 | | Total work load | | | 235 |
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