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| BTBL5430 | Techniques in Microbiology | 3+0+0 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of FISHERIES TECHNOLOGY ENGINEERING | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Lab work | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | -- | | Co-Lecturer | Prof. Dr. Erol Çapkın | | Language of instruction | | | Professional practise ( internship ) | None | | | | The aim of the course: | | Students will use advance microbiology techniques to identify pathogens |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | learn biochemical profile-based microbial identification systems | 2,5,6,10,13 | 1,5,6 | | PO - 2 : | learn pulsed-field gel electrophoresis | 5,10 | 1,5,6 | | PO - 3 : | learn in vitro nucleic acid amplification | 2,5,6,10,13,14 | 1,5,6 | | PO - 4 : | learn PCR and its variations | 2,5,6,10,13 | 1,5,6 | | PO - 5 : | learn direct nucleotide sequencing for amplification product identification | 2,5,6,10 | 1,5,6 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| Identification and classification of fish pathogens from water and fish, determinations of aseptic techniques; procedures and methods for isolation, characterization and identification of fish pathogens. Introduction to current recombinant techniques used to identify fish pathogens. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Biochemical profile-based microbial identification systems | | | Week 2 | probe-based microbial detection and ıdentification | | | Week 3 | Pulsed-field gel electrophoresis
| | | Week 4 | in vitro nucleic acid amplification
| | | Week 5 | PCR and ıts variations
| | | Week 6 | Non?polymerase chain reaction mediated target amplification techniques
| | | Week 7 | Recent advances in probe amplification technologies
| | | Week 8 | Mid-term exam | | | Week 9 | Detection and characterization of molecular amplification products: agarose gel electrophoresis, southern blot hybridization, restriction enzyme digest analysis, and enzyme-linked ımmunoassay | | | Week 10 | Direct nucleotide sequencing for amplification product ıdentification
| | | Week 11 | Microarray-based microbial ıdentification and characterization
| | | Week 12 | Diagnostic microbiology using real-time pcr based technology
| | | Week 13 | Bacterial ıdentification based on 16s ribosomal rna gene sequence analysis
| | | Week 14 | Tekrarlı sekans tabanlı PCR ile moleküler strain tiplemeleri | | | Week 15 | Homework presentation | | | Week 16 | End-of-term exam | | | |
| 1 | Tang, Y.W. and Stratton, C.W. 2006; Advanced Techniques in Diagnostic Microbiology. Springer Science and Business Media, LLC, New York, NY, USA. | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | 20/11/2018 | 2 | 25 | | Presentation | 10 | 11/12/2018 | 1 | 25 | | End-of-term exam | 17 | 08/01/2019 | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 8 | 12 | 96 | | Arasınav için hazırlık | 10 | 1 | 10 | | Arasınav | 2 | 1 | 2 | | Uygulama | 4 | 5 | 20 | | Ödev | 10 | 2 | 20 | | Dönem sonu sınavı için hazırlık | 10 | 1 | 10 | | Dönem sonu sınavı | 2 | 1 | 2 | | Total work load | | | 202 |
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