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| SECB445 | Detern. and Charc. of Biomolecules | 4+0+0 | ECTS:4 | | Year / Semester | Fall Semester | | Level of Course | First Cycle | | Status | Elective | | Department | DEPARTMENT of CHEMISTRY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 4 hours of lectures per week | | Lecturer | -- | | Co-Lecturer | Lecturers in Biochemistry Division | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | The Students Should Obtain Knowledge of Approaches and Methods in Determinations, Both Qualitative and Quantitative, and Characterization of Biomolecules, and Learn the Applications in Medicine and Industry. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | know the applications of instrumental methods used for determination and biomolecules. | 2,3,4,6,7 | 1 | | LO - 2 : | know how to perform the characterization of biomolecules. | 2,3,4,6,7 | 1 | | LO - 3 : | describe the methods used for clinical and industrial investigation. | 2,3,4,6,7 | 1 | | LO - 4 : | know chromatographic applications. | 2,3,4,6,7 | 1 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Classes of Biomolecules. Structures, Functions, and Localization of Nucleotides and Nucleic Acids. Determination of Nucleic Acids, Sequence Analysis. Detection of Genes, Polymerase Chain Reaction (PCR) and Applications. DNA Analyses in Medicine, Molecular Weight Determinations, DNA Electrophoresis, Genomic Investigations. Amino Acids, Classes, Structures, Functions and Localizations of Proteins. Protein Determinations, Amino Acid Sequence Analysis, Protein Electrophoresis, Proteomics Investigations. Enzymes, Determinations of Their Concentrations and Activities. Enzyme Analyses in Medicine and Industry. Immunochemical Methods. Classification, Structures, Functions and Localizations of Carbohydrates. Carbohydrate Determination Methods. Carbohydrate Analyses in Medicine, Industry and Quality Control. Classification, Structures, Functions and Localizations of Lipids. Lipid Analyses. Animal and Plant Hormones and Related Tests. Determinations of Vitamins and Metabolites. Sample Preparation Methods for Various Sample Types. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Classes of Biomolecules. Structures, Functions, and Localization of Nucleotides and Nucleic Acids. Determination of Nucleic Acids, Sequence Analysis. | | | Week 2 | Detection of Genes, Polymerase Chain Reaction (PCR) and Applications. | | | Week 3 | DNA Analyses in Medicine, Molecular Weight Determinations, DNA Electrophoresis, Genomic Investigations. | | | Week 4 | Amino Acids, Classes, Structures, Functions and Localizations of Proteins. | | | Week 5 | Protein Determinations, Amino Acid Sequence Analysis, Protein Electrophoresis, Proteomics Investigations. | | | Week 6 | Enzymes, Determinations of Their Concentrations and Activities. | | | Week 7 | Enzyme Analyses in Medicine and Industry. Immunochemical Methods. | | | Week 8 | Mid-term exam | | | Week 9 | Classification, Structures, Functions and Localizations of Carbohydrates. | | | Week 10 | Carbohydrate Determination Methods. Carbohydrate Analyses in Medicine, Industry and Quality Control. | | | Week 11 | Classification, Structures, Functions and Localizations of Lipids. Lipid Analyses. | | | Week 12 | Animal and Plant Hormones and Related Tests. | | | Week 13 | Determinations of Vitamins and Metabolites. | | | Week 14 | 2nd midexam | | | Week 15 | Sample Preparation Methods for Various Sample Types. | | | Week 16 | End-of-term exam | | | |
| 1 | Güner, S. (Ed.) 2001; Temel Biyokimya Teknikleri ve Uygulamaları, Meriç Kırtasiye, Trabzon | | | |
| 1 | Klug, W.S. ve Cummings, M.R. (Cihan Öner, çeviri ed.) 2002, Palme yayıncılık, Ankara | | | 2 | Farrel, S.O. and Ranallo, R.T. 2000; Experiments in Biochemistry, Saunders Publishing, FL | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | 11/11/2010 | 1,25 | 30 | | In-term studies (second mid-term exam) | 12 | 16/12/2010 | 1,25 | 20 | | End-of-term exam | 16 | 10/01/2011 | 1,25 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | | | | |
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