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| SECB443 | Enzymes and Enzyme Kinetics | 4+0+0 | ECTS:4 | | Year / Semester | Fall Semester | | Level of Course | First Cycle | | Status | Elective | | Department | DEPARTMENT of CHEMISTRY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 4 hours of lectures per week | | Lecturer | -- | | Co-Lecturer | Lecturers in Biochemistry Division | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | The students should obtain knowledge about important of enzymes and how they can use industry and biotechnology. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | have knowledge about protein function and structure. | 1,3,8 | 1 | | LO - 2 : | have knowledge about stereospecifitiy of enzymes and will be able to classify of enzymes | 1,2,4 | 1 | | LO - 3 : | calculate and comment kinetics of enzymes having mono and polisubstrate | 1,2,3,4 | 1,3 | | LO - 4 : | have knowledge about regulation, mechanism and industrial apply of enzyme | 1,2,4 | 1,3 | | LO - 5 : | comprehend protein purification techniques and apllications | 1,2,4,5,7,8 | 1,5,6 | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| History of enzyme, introduction to enzyme and aminoacids, protein structure. Clasification of Enzymes. Monomeric and oligomeric enzymes. Stereospesifisity of enzyme. Catalayse and introduction to kinetic. Kinetics of enzyme having monosubstrate and polisubstrate (Michealis-Menten, Lineweaver-Burk, Edia-Hofsttee equations and curves) . Regulation of enzyme activitiy and inhibition kinetics. Analysis of active site conformation. Kinetics of biological macromolecules and interaction of biological macromolecules. Enzyme purification techniques. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Enzyme history | | | Week 2 | Structures of amino acids and proteins | | | Week 3 | Functions of proteins | | | Week 4 | Protein-ligand interactions | | | Week 5 | Enzyme classification; monomeric and Oligomeric enzymes | | | Week 6 | Enzyme specificity, introduction to catalysis and kinetics | | | Week 7 | Enzymatic catalysis mechanisms | | | Week 8 | Mid-term exam | | | Week 9 | Kinetics of single and multi substrate enzymes (Michealis-Menten, Lineweaver-Burk, Edia-Hofsttee equations and graphics) | | | Week 10 | Enzyme inhibitions, inhibition types | | | Week 11 | Ernzyme regulations and inhibition kinetics | | | Week 12 | Investigating the active site of enzymes | | | Week 13 | Assignments and presentations | | | Week 14 | Enzyme purification techniques | | | Week 15 | Enzyme characterization | | | Week 16 | End-of-term exam | | | |
| 1 | Dr. Trevor Palmer, Edit.; Çev: Salih Cengiz, Müjgan Cengiz, 1994; Enzim Bilgisi (Understanding Enzymes) Bilimsel ve Teknik Yayınları, İstanbul. | | | 2 | David Nelson, Michael M.Cox; Nedret Kılıç (Çev. Ed.), 2005; Lehninger Biyokimyanın İlkeleri, Ankara, 3. Baskıdan Çeviri. | | | |
| 1 | E.Edip Keha, Ö. İrfan Küfrevioğlu, 2006; Biyokimya, Aktif Yayınevi, İstanbul. | | | 2 | Saadettin Güner, 2007; Biyokimya-I, Trabzon | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | 10/11/2010 | 1 | 30 | | Presentation | 13 | 13/12/2010 | 1 | 20 | | End-of-term exam | 16 | 08/01/2011 | 1,5 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 4 | 14 | 56 | | Sınıf dışı çalışma | 1 | 14 | 14 | | Arasınav için hazırlık | 8 | 1 | 8 | | Arasınav | 2 | 1 | 2 | | Ödev | 2 | 4 | 8 | | Dönem sonu sınavı için hazırlık | 10 | 1 | 10 | | Dönem sonu sınavı | 2 | 1 | 2 | | Total work load | | | 100 |
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