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| EBK6012 | Enzyme Kinetics | 3+0+0 | ECTS:7.5 | | Year / Semester | Spring Semester | | Level of Course | Third Cycle | | Status | Elective | | Department | DEPARTMENT of BIOCHEMISTRY (PHARMACY) | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 3 hours of lectures per week | | Lecturer | Prof. Dr. Arzu ÖZEL | | Co-Lecturer | - | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Will be able to understand behavior of kinetics of enzymes. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Will be able to understand about the basics of an enzyme catalyzed reaction | 1,2 | 1,5, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| The kinetic mechanism and kinetic behavioral models of an enzymatic reaction, calculation of the kinetic constants of an enzyme catalyzed reaction. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Factors affecting the rate of enzymatic reaction-I: enzyme, substrate concentration, temperature and pH of the experimental media | | | Week 2 | Factors affecting the rate of enzymatic reaction-II: enzyme, substrate concentration, temperature and pH of the media: reversible, irreversible inhibitors, activators | | | Week 3 | Rapid equilibrium steady-state approaches | | | Week 4 | Michaelis-Menten and Lineweaver-Burk plots | | | Week 5 | Hanes-Woolf, Woolf-Augustinsson-Hofstee plots | | | Week 6 | Eadie-Scatchard plots | | | Week 7 | Lee-Wilson plot, Dixon plot | | | Week 8 | Inhibition Kinetics I-Cleland nomenclature, competitive, noncompetitive inhibition | | | Week 9 | Midterm | | | Week 10 | Inhibition kinetics II-unkompetitif, partial competitive, noncompetitive partial inhibition | | | Week 11 | Inhibition kinetics III-linear and hyperbolic mixed inhibition kinetics | | | Week 12 | midterm exam | | | Week 13 | Multisubsrat reactions: sequential | | | Week 14 | Multisubsrat reactions II: ping pong, Theorell-Chance mechanism | | | Week 15 | Preparation for the exam | | | Week 16 | Final | | | |
| 1 | Gürdal Figen, Ademoğlu Evin. 2010; Ankara, Nobel Tıp kitapevi Biyokimya, | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 1 | 22/04/2024 | 2 | 25 | | Homework/Assignment/Term-paper | 1 | 26/05/2024 | 2 | 25 | | End-of-term exam | 1 | 08/06/2024 | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 3 | 14 | 42 | | Arasınav için hazırlık | 3 | 2 | 6 | | Arasınav | 2 | 2 | 4 | | Ödev | 3 | 1 | 3 | | Dönem sonu sınavı | 3 | 1 | 3 | | Total work load | | | 100 |
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