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| EFG5100 | Phytochemical Analysis-1 | 3+0+3 | ECTS:7.5 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Compulsory | | Department | DEPARTMENT of PHARMACOGNOSY | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 3 hours of lectures and 3 hours of laboratory per week | | Lecturer | -- | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | This course provides basic knowledge and ability about classification of secondary metabolites and procedures for isolation, purification and quantitative analysis of secondary metabolites for the development herbal medicine from natural sources. |
| Programme Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | PO - 1 : | Prepare different polarity extracts.
| 1,2,10,12 | 1,3,5, | | PO - 2 : | Perform isolation techniques. | 1,2,10,12 | 1,3,5, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), PO : Learning Outcome | | |
| This course includes classification of secondary metabolites in natural resources, also chromatographic and spectroscopic techniques used in isolation, extraction, structure elucidation and quantitative analysis of secondary metabolites |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Definition of cheromatography | | | Week 2 | Chromatography techniques | | | Week 3 | Chromatography techniques | | | Week 4 | Thin Layer Chromatography | | | Week 5 | Thin layer chromatography practice | | | Week 6 | Column chromatography | | | Week 7 | Column chromatography practice | | | Week 8 | Mid-term exam | | | Week 9 | Solvent selection | | | Week 10 | Overview of structural systems | | | Week 11 | Revelators | | | Week 12 | Medium and high pressure chromatography | | | Week 13 | Medium and high pressure chromatography | | | Week 14 | Other chromatography techniques | | | Week 15 | Electrophoresis applications at phytochemical analysis | | | Week 16 | Exam | | | |
| 1 | A.J. Harborne , Phytochemical Methods, Springer; 3rd edition (1998). | | | 2 | Monika Waksmundzka-Hajnos, Joseph Sherma, Teresa Kowalska, Thin Layer Chromatography in Phytochemistry (Chromatographic Science Series), CRC Press; 1 edition (March 4, 2008). | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 8 | 18/11/2021 | 1 | 30 | | Practice | 1
14 | | 1 | 10 | | Presentation | 14 | 03/01/2022 | 1 | 10 | | End-of-term exam | 14 | 06/01/2022 | 2 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 3 | 14 | 42 | | Sınıf dışı çalışma | 7 | 14 | 98 | | Laboratuar çalışması | 3 | 14 | 42 | | Arasınav için hazırlık | 2 | 1 | 2 | | Arasınav | 1 | 1 | 1 | | Uygulama | 0 | 0 | 0 | | Proje | 0 | 0 | 0 | | Dönem sonu sınavı için hazırlık | 2 | 2 | 4 | | Dönem sonu sınavı | 2 | 1 | 2 | | Total work load | | | 191 |
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