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| BIY2027 | CELL BIOLOGY LABORATORY | 0+0+2 | ECTS:2 | | Year / Semester | Fall Semester | | Level of Course | First Cycle | | Status | Compulsory | | Department | DEPARTMENT of BIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 2 hours of laboratory per week | | Lecturer | Prof. Dr. Zihni DEMİRBAĞ | | Co-Lecturer | PROF. DR. İsmail DEMİR, Prof. Dr. Remziye NALÇACIOĞLU, | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | are to observe sub units and understand their functions of cells. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | Have knowledge about various aspects of cells in detail | 1,3,8 | 4, | | LO - 2 : | Have experience with laboratory techniques | 1,3,8 | 4, | | LO - 3 : | Discus several current topics in hücre biyolojisi with his/her classmates | 1,3,8 | 4, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Introduction to cell biology laboratory; Microscopy and microscope techniques and diversity of cells; Microscopic measurement units and measuring under microscope; Sectioning, staining and permanent sample preparation; Microscopic observation of sub cellular structures; Cell culture techniques; pH and biological buffers; Centrifuges and centrifugation; Isolation and analysis of sub cellular structures, nucleic acids and proteins; Enzymes and their activities; Cell division and chromosome morphologies; Transport of cellular molecules from membrane; Photosynthesis and respiration
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Introduction to cell biology laboratory | | | Week 2 | Microscopy and microscope techniques and diversity of cells | | | Week 3 | Microscopic measurement units and measuring under microscope
| | | Week 4 | Sectioning, staining and permanent sample preparation
| | | Week 5 | Microscopic observation of sub cellular structures | | | Week 6 | Cell culture techniques | | | Week 7 | pH and biological buffers | | | Week 8 | Centrifuges and centrifugation | | | Week 9 | Mid-term exam | | | Week 10 | Isolation and analysis of sub cellular structures, nucleic acids and proteins
| | | Week 11 | Enzymes and their activities
| | | Week 12 | Homework return | | | Week 13 | Cell division and chromosome morphologies | | | Week 14 | Transport of cellular molecules from membrane
| | | Week 15 | Photosynthesis and respiration | | | Week 16 | End-of-term exam | | | |
| 1 | Demirbag, Z. 2009. Hücre Biyolojisi Laboratuar Klavuzu. Karadeniz Teknik Üniversitesi, Trabzon
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| 1 | Heidcamp, W. H. 1995. Cell Biology Laboratory Manual. Saint Peter, Minnesota, USA | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 25/11/2021 | 1 | 50 | | End-of-term exam | 16 | 21/1/2022 | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 0 | 0 | 0 | | Sınıf dışı çalışma | 1 | 15 | 15 | | Laboratuar çalışması | 2 | 15 | 30 | | Arasınav için hazırlık | 0 | 8 | 0 | | Arasınav | 2 | 1 | 2 | | Uygulama | 0 | 0 | 0 | | Klinik Uygulama | 0 | 0 | 0 | | Ödev | 0 | 15 | 0 | | Proje | 0 | 0 | 0 | | Kısa sınav | 0 | 0 | 0 | | Dönem sonu sınavı için hazırlık | 0 | 15 | 0 | | Dönem sonu sınavı | 2 | 1 | 2 | | Diğer 1 | 6 | 1 | 6 | | Diğer 2 | 5 | 1 | 5 | | Total work load | | | 60 |
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