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| BIY4005 | Molecular Biology Lab | 0+0+2 | ECTS:4 | | Year / Semester | Fall Semester | | Level of Course | First Cycle | | Status | Compulsory | | Department | DEPARTMENT of BIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face, Group study, Lab work , Practical | | Contact Hours | 14 weeks - 2 hours of laboratory per week | | Lecturer | Prof. Dr. Ali Osman BELDÜZ | | Co-Lecturer | PROF. DR. Sabriye ÇANAKÇI, | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Laboratory courses and exercises aim to equip the student with basic molecular biology techniqes and use modern techniques to reinforce Molecular Biology lecture material. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | Isolate and safely handle nucleic acids in the laboratory | 1,4,6 | 1,3,4, | | LO - 2 : | Explain principles of analyses methods of nucleic acids | 1,4,6 | 1,3,4, | | LO - 3 : | Perform basic nucleic acid manipulations | 1,4,6 | 1,2,4, | | LO - 4 : | be familiar with basic molecular biology lab safety protocols. | 1,4,6 | 1,2,4, | | LO - 5 : | understand and explain the use of modern molecular biology techniques. | 1,4,6 | 1,3,4, | | LO - 6 : | communicate clearly and effectively oraly or through the written word by reporting the results of their research or laboratory work | 1,4,6 | 1,3,4, | | LO - 7 : | apply a basic laboratory knowledge of molecular biology to the study of biomolecules, cellular processes, and disease. | 1,4,6 | 1,3,4, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Preparation of bacterial medium and bacterial culture, screening of antibiotic resistant genes in bacteria, isolation of genomic and plasmid DNA, isolation of protein, determination of quantity and purity of nucleic acids and proteins, electrophoresis of nucleic acids and proteins, restriction endonucleases and their usage, gene cloning, transformation and expression, polimerase chain reaction and applications.Laboratory exercises may require additional time outside of the scheduled lab hours. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Lab safety and adherence to national health guidelines | | | Week 2 | Mesaurements, Micropipeting and sterile techniques | | | Week 3 | bacterail medium preparation ; bacterial culture techniques | | | Week 4 | Plasmid isolation | | | Week 5 | Assay for an antibiotic resistance enzyme | | | Week 6 | Isolation of proteins; determination of protein concentraion | | | Week 7 | Analysis of SDS-PAGE | | | Week 8 | DNA restriction analysis | | | Week 9 | Mid-term exam | | | Week 10 | Preparation of recombinant DNA | | | Week 11 | Preparation of competent cells | | | Week 12 | Transformation of E.coli with recombinant DNA | | | Week 13 | Selection and isolation of recombinant DNA | | | Week 14 | Digestion of Recombinant DNA and control of digestion | | | Week 15 | DNA Polymorphisms assays by PCR | | | Week 16 | End-of-term exam | | | |
| 1 | Molecular Biology Laboratory Notes, Belduz vd. 2008, Trabzon | | | |
| 1 | Watson, James D., Tania A. Baker, Stephen P. Bell, and Alexander Gann (2007), Molecular Biology of the Gene. Sixth Edition. San Francisco, CA: Benjamin Cummings | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Laboratory exam | 9 | 24/11/2021 | 1 | 40 | | Homework/Assignment/Term-paper | 15 | 08/01/2022 | 1 | 10 | | End-of-term exam | 16 | 18/01/2022 | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 0 | 0 | 0 | | Sınıf dışı çalışma | 2 | 20 | 40 | | Laboratuar çalışması | 2 | 15 | 30 | | Arasınav için hazırlık | 2 | 10 | 20 | | Arasınav | 1 | 1 | 1 | | Ödev | 1 | 1 | 1 | | Dönem sonu sınavı için hazırlık | 2 | 10 | 20 | | Dönem sonu sınavı | 1 | 1 | 1 | | Diğer 1 | 1 | 6 | 6 | | Diğer 2 | 1 | 1 | 1 | | Total work load | | | 120 |
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