|
|
| BIY2014 | CELL BIOCEMISTRY LABORATORY | 0+0+2 | ECTS:2 | | Year / Semester | Spring Semester | | Level of Course | First Cycle | | Status | Compulsory | | Department | DEPARTMENT of BIOLOGY | | Prerequisites and co-requisites | None | | Mode of Delivery | Face to face | | Contact Hours | 14 weeks - 2 hours of laboratory per week | | Lecturer | Prof. Dr. İsmail DEMİR | | Co-Lecturer | PROF. DR. Faik Ahmet AYAZ,PROF. DR. İsmail DEMİR,PROF. DR. İsmail DEMİR, | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Methods for preparing solutions as well as having bacground on the methods and equipments employed in the qualitative and queantitative determination of biomolecules are the main goals of the course |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | to have background how to prepare solutions and concentration units | 8 | | | LO - 2 : | to have basic knowledge concerning qualitative and quantitative analyses of biomolecules | 8 | | | LO - 3 : | to have an ability in terms of using basic instruments available in biochemistry labarotory | 8 | | | LO - 4 : | to be able to evaluate qualitative and quantitative analyses data. | 8 | | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| General rules, introducing materials and chemicals being used in biochemistry laboratory, Preparing solutions and concentration units (%, molarity, normality, pp), Acids,-bases and buffers, Titration:, Acid-base titration, Amino acid titration, , Thin Layer Chromatography, Spectrophotometry and calibration graphics, Quantitative protein determination and Bradford method, Amino acid precipitation reactions and preciptation of proteins, Qualitative carbohydrate determination, Saponification reactions and Soap production, Protein purification techniques, Ion exchange column chromatoğrapy, Isolation of soluble proteins, Structures and classification of enzymes, Enzyme activity tests, , estimation of enzyme units, calculation of free fatty acids. |
| |
| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | General rules, Introduction to materials and chemicals being used in biochemistry laboratory | | | Week 2 | Preparing solutions and concentration units (%, molarity, normality, ppm),
| | | Week 3 | Acids,-bases and buffers | | | Week 4 | Titration:, Acid-base titration, Amino acid titration | | | Week 5 | Thin Layer Chromatography | | | Week 6 | Spectrophotometry and calibration graphics | | | Week 7 | Quantitative protein determination and Bradford method | | | Week 8 | Amino acid precipitation reactions and preciptation of proteins | | | Week 9 | MID-TERM EXAM | | | Week 10 | Qualitative carbohydrate determination | | | Week 11 | Saponification reactions and Soap production | | | Week 12 | Protein purification techniques, Ion exchange column chromatography | | | Week 13 | Isolation of soluble proteins | | | Week 14 | Structures and classification of enzymes, Enzyme activity tests, estimation of enzyme units | | | Week 15 | FAts and Oils; calculation of free fatty acid | | | Week 16 | FINAL EXAM | | | |
| 1 | Aktümsek, A. Nurullahoğlu, Z. Pratik Biyokimya Kitabı, Nobel yayın dağıtım, 2. Baskı) | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 21/04/2022 | 1 | 50 | | End-of-term exam | 16 | 15/06/2022 | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Laboratuar çalışması | 2 | 14 | 28 | | Arasınav için hazırlık | 4 | 5 | 20 | | Arasınav | 1 | 1 | 1 | | Kısa sınav | .5 | 14 | 7 | | Dönem sonu sınavı için hazırlık | 3 | 1 | 3 | | Dönem sonu sınavı | 1 | 1 | 1 | | Total work load | | | 60 |
|