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| ECZ2036 | Research Techniques in Biochemistry | 2+0+0 | ECTS:4 | | Year / Semester | Spring Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of PHARMACEUTICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 2 hours of lectures per week | | Lecturer | Doç. Dr. Burak BARUT | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | |
To teach the basic methods used for research in biochemistry |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | Recognizes materials and reagents used in biochemistry, can prepare buffer compounds, has information about spectrophotometer, cell culture, western-southern-northern blotting-flow cytometry, protein and DNA purification methods. | 1,2,4 | 1, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| It is aimed to explain the necessary basic techniques in order to be able to carry out research and development activities in the field of biochemistry. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Literature Review in Biochemistry | | | Week 2 | Preparation of Acid, Base and Buffer Solutions | | | Week 3 | Spectrophotometric Methods | | | Week 4 | Spectrophotometric Methods | | | Week 5 | Electrophoretic Methods (Protein) | | | Week 6 | Electrophoretic Methods (DNA) | | | Week 7 | Cell Cultures | | | Week 8 | Cell Cultures | | | Week 9 | Midterm | | | Week 10 | Western Blotting | | | Week 11 | Southern ve Northtern Blotting | | | Week 12 | Elisa Technique | | | Week 13 | Flow Sitometry | | | Week 14 | Protein Purification Techniques | | | Week 15 | DNA Purification Techniques | | | Week 16 | Final exam | | | |
| 1 | Robyt,JF., White BJ.1987;Biochemical Techniques,Waveland Press, Illinois | | | 2 | Boyer, RF.1993;Modern Experimental Biochemistry, The Benjamin /Cummings Publ.,California
| | | 3 | Barker,K.1998;At The Bench, Cold Spring harbor Lab Press, New York | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 15.04.2024 | | 50 | | End-of-term exam | 16 | 03.06.2024 | | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 14 | 28 | | Sınıf dışı çalışma | 2 | 14 | 28 | | Laboratuar çalışması | 1 | 14 | 14 | | Arasınav için hazırlık | 3 | 7 | 21 | | Arasınav | 3 | 1 | 3 | | Dönem sonu sınavı için hazırlık | 4 | 2 | 8 | | Dönem sonu sınavı | 3 | 1 | 3 | | Total work load | | | 105 |
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