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| ECZ5009 | Plant Tissue Culture in Pharmacy | 2+0+0 | ECTS:4 | | Year / Semester | Fall Semester | | Level of Course | Second Cycle | | Status | Elective | | Department | DEPARTMENT of PHARMACEUTICS | | Prerequisites and co-requisites | None | | Mode of Delivery | | | Contact Hours | 14 weeks - 2 hours of lectures per week | | Lecturer | Dr. Öğr. Üyesi Nurdan YAZICI BEKTAŞ | | Co-Lecturer | | | Language of instruction | Turkish | | Professional practise ( internship ) | None | | | | The aim of the course: | | Extensive information will be given about the basics, types and applications of plant cell and tissue culture, and theoretical information will be given on how it can be used in secondary metabolite production, and the advantages and disadvantages of the subject in this field will be discussed. |
| Learning Outcomes | CTPO | TOA | | Upon successful completion of the course, the students will be able to : | | | | LO - 1 : | Will be able to determine the necessary conditions for culturing plant tissues. | 2,3,5,8 | 1,5, | | LO - 2 : | Will have knowledge about tissue culture techniques. | 2,3,5,8 | 1,5, | | LO - 3 : | Will have information about secondary metabolite production by tissue culture method in plants. | 2,3,5,8 | 1,5, | | CTPO : Contribution to programme outcomes, TOA :Type of assessment (1: written exam, 2: Oral exam, 3: Homework assignment, 4: Laboratory exercise/exam, 5: Seminar / presentation, 6: Term paper), LO : Learning Outcome | | |
| Definition of plant tissue culture, application areas, requirements of tissue culture laboratory (medium and culture conditions, sterilization techniques), tissue culture types (callus, cell suspension, anther/microspore, protoplast, embryo, meristem), in vitro plant regeneration methods (organogenesis, somatic embryogenesis), secondary metabolite production (basic principles, necessary conditions, economic importance), use and importance of bioreactors, summary of current studies are given. |
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| Course Syllabus | | Week | Subject | Related Notes / Files | | Week 1 | Plant Tissue Culture: Plant life circle | | | Week 2 | Plant tissue culture: Historical development and application areas | | | Week 3 | Basic concepts used in plant tissue and cell cultures | | | Week 4 | Plant tissue culture basic techniques, setup a culture laboratory | | | Week 5 | Sterilization techniques, media components and preparation | | | Week 6 | Culture types (Callus, cell suspension, Anther/microspore, protoplast, embryo, meristem) | | | Week 7 | Organogenesis: Explant selection, nutrient media contents and culture conditions, Organogenesis varieties and its success in various species | | | Week 8 | Somatic embryogenesis, explant source, investigation of effective factors, usage areas, clonal propagation and synthetic seed production | | | Week 9 | Midterm | | | Week 10 | Protoplast culture and Somatic hybridization; totipotency and definitions, Structure of plant cell wall, isolation of protoplast, factors that ensure the success of protoplast culture | | | Week 11 | Haploid plant production from anther culture and microspore culture | | | Week 12 | Basic principles of secondary metabolite production and necessary conditions | | | Week 13 | Economic importance of secondary metabolite production | | | Week 14 | Use of bioreactor; advantages and disadvantages | | | Week 15 | Reviewing current articles | | | Week 16 | Final Exam | | | |
| 1 | Öktem, Hüseyin Avni, Yücel, Meral, Ed. Stewart, C. Neal. 2016; Bitki Biyoteknolojisi ve Genetik: İlkeler, Teknikler ve Uygulamalar, Nobel Akademik Yayıncılık Eğitim Danışmanlık Tic. LTD. ŞTİ, No:280, Ankara | | | |
| 1 | Neumann, Karl-Hermann, Kumar, Ashwani, Imani, Jafargholi. 2009; Plant Cell and Tissue Culture - A Tool in Biotechnology: Basics and Application, Springer-Verlag Berlin Heidelberg, Germany | | | |
| Method of Assessment | | Type of assessment | Week No | Date | Duration (hours) | Weight (%) | | Mid-term exam | 9 | 19.11.2023 | 1 | 25 | | Presentation | 15 | 08.01.2024 | 1 | 25 | | End-of-term exam | 16 | 15.01.2024 | 1 | 50 | | |
| Student Work Load and its Distribution | | Type of work | Duration (hours pw) | No of weeks / Number of activity | Hours in total per term | | Yüz yüze eğitim | 2 | 14 | 28 | | Arasınav için hazırlık | 8 | 1 | 8 | | Arasınav | 1 | 1 | 1 | | Ödev | 2 | 1 | 2 | | Dönem sonu sınavı için hazırlık | 15 | 1 | 15 | | Dönem sonu sınavı | 1 | 1 | 1 | | Total work load | | | 55 |
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